HISTONES FROM DIABETIC RATS CONTAIN INCREASED LEVELS OF ADVANCED GLYCATION END-PRODUCTS
HISTONES FROM DIABETIC RATS CONTAIN INCREASED LEVELS OF ADVANCED GLYCATION END-PRODUCTS
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DOI：
10.1006/bbrc.1995.1935
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发表时间：
1995-07-06
影响因子：
3.1
通讯作者：
BENDAYAN, M
中科院分区：
文献类型：
--
作者：
GUGLIUCCI, A;BENDAYAN, M
在最近的一份报告中，我们已经证明了在体外形成的晚期糖基化终产物（AGEs）在组蛋白上的时间和糖浓度依赖的方式。在目前的工作中，我们在活体中检查了组蛋白晚期糖基化。用链脲佐菌素诱导大鼠糖尿病，并观察其高血糖状态24周。糖尿病大鼠的血浆蛋白和血红蛋白中有早期糖基化产物的积累，糖尿病大鼠肝脏中的组蛋白显示AGEs水平比同龄对照组高3倍。组蛋白AGEs随糖尿病病程的延长而增加，并有随年龄增加的趋势。类似的趋势在皮肤胶原蛋白中也很明显。我们的数据表明，糖尿病诱导组蛋白上AGE产物的积累增加。这加强了细胞内蛋白发生晚期糖基化的概念，并提示细胞内糖基化在糖尿病相关的治疗过程中可能发挥的作用。(C) 1995学术出版社，Inc。
In a recent report we have demonstrated the in vitro formation of advanced glycation end products (AGEs) on histones in a time and sugar concentration dependent fashion. In the present work we examined histone advanced glycation in vive. Diabetes was induced in rats by streptozotocin injection and the hyperglycemic state was maintained and surveyed for up to 24 weeks. Diabetic rats showed accumulation of early glycation products in plasma proteins and in hemoglobin, Histones from the liver of diabetic rats showed AGEs levels three-fold higher than those of their age-matched controls. Histone AGEs increased with the duration of diabetes and tended to increase with the age as well. Similar tendencies were apparent in skin collagen. Our data demonstrate that diabetes induces an increase in the accumulation of AGE products on histones. This reinforces the concept that advanced glycation occurs in intracellular proteins and suggests a possible role for intracellular glycation in the increased theratogeny associated with diabetes mellitus. (C) 1995 Academic Press, Inc.