Autophagic and Apoptotic Cell Death in Amniotic Epithelial Cells
Autophagic and Apoptotic Cell Death in Amniotic Epithelial Cells
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DOI：
10.1016/j.placenta.2008.09.001
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发表时间：
2008-11-01
期刊：
影响因子：
3.8
通讯作者：
Xu, L. -Y.
中科院分区：
文献类型：
--
作者：
Shen, Z. -Y.;Li, E. -M.;Xu, L. -Y.
本研究旨在探讨自噬性细胞死亡是否与细胞凋亡有关，以及自噬性细胞死亡是否参与了足月羊膜破裂的发生过程。收集40个新鲜足月胎盘，其中20个来自胎盘边缘附近的位置，20个来自足月妊娠胎盘自然破裂部分的边缘。用扫描电镜（SEM）和透射电镜（TEM）观察羊膜上皮（AE）细胞。用激光共聚焦显微镜（LSCM）或流式细胞仪检测细胞自噬和凋亡。免疫印迹法检测BCL 2和BAX。扫描电镜下羊膜上皮在胎盘附近正常。他们有一个萎缩的外观在其自然断裂部分的边缘。AE细胞中PCD可分为三种类型：自噬性细胞死亡，MDC和PI染色阳性;凋亡性细胞死亡;混合型。定量检测显示，破裂部位附近的AE细胞比胎盘附近的AE细胞有更多的死亡细胞，包括自噬细胞和凋亡细胞。BAX蛋白表达增加，BCL 2蛋白表达减少。内源性细胞凋亡和自噬性细胞死亡是AE细胞的基本事件，它们参与了足月妊娠人羊膜破裂的发生。(C)2008爱思唯尔有限公司保留所有权利。
The aim of this paper is to determine if autophagic cell death is associated with apoptosis and whether it participates in the process of term amniotic rupture. Forty pieces of fresh term amnions, including twenty from a position near the margin of the placentas and twenty from the margin of the naturally ruptured part of the placentas in term gestation were collected, respectively. The amnions were examined by scanning electron microscopy (SEM) and amniotic epithelial (AE) cells were examined by transmission electron microscopy (TEM). Autophagic and apoptotic cell death (PCD) were assayed by laser scanning confocal microscopy (LSCM) or flow cytometry using monodansylcadaverin (MDC) and propidium iodide (PI) stain. BCL2 and BAX were examined by immunoblotting. Under SEM the amniotic epithelia appeared normal in the position near the placenta. They had an atrophied appearance in the margin of their natural broken parts. In the AE cells PCD was divided into three subtypes by TEM: autophagic cell death with positive stains of MDC and PI; apoptotic cell death: and the mixed type. Quantitative detection showed that there were more death cells, including autophagic and apoptotic, in the AE cells near the ruptured parts than near the placentas. An increased expression of BAX and a decreased expression of BCL2 protein in the AE cells near the broken margin were observed. Apoptotic and autophagic cell death by the intrinsic pathway are the basic event in the AE cell and they are involved in the cause of membrane rupture of the human amnion in term gestation. (C) 2008 Elsevier Ltd. All rights reserved.