Human Corneal Basal Epithelial Cells Express an Embryonic Stem Cell Marker OCT4
Human Corneal Basal Epithelial Cells Express an Embryonic Stem Cell Marker OCT4
复制标题
DOI：
10.3109/02713683.2010.516465
复制
发表时间：
2010-10
影响因子：
2
通讯作者：
Shi-You Zhou;Cheng Zhang;Ebrahimi Baradaran;R. Chuck
中科院分区：
文献类型：
--
作者：
Shi-You Zhou;Cheng Zhang;Ebrahimi Baradaran;R. Chuck
目的：转录因子作为分子开关激活或抑制特定基因，从而控制细胞表型。转录因子OCT 4对胚胎干细胞的自我更新和维持至关重要。在体外和体内，OCT 4表达在所有分化的体细胞类型中下调。我们的目的是研究人角膜上皮是否表达OCT 4。材料与方法：通过荧光免疫组织化学检测9例人角膜标本和人睾丸腺瘤阳性对照样本中的OCT 4蛋白。研究了人角膜上皮细胞和人胚胎干细胞（ESC）中的OCT 4 mRNA。RT-PCR产物经DNA测序分析。结果：所有角膜均显示核染色，仅限于角膜上皮基底层。OCT 4阳性染色细胞主要分布于角膜缘上皮基底层，但也有部分分布于角膜缘周围和中央上皮基底层。OCT 4 mRNA在所有人角膜上皮和人ESC细胞中检测到。阴性对照中未检测到OCT 4 mRNA或蛋白。测序结果表明，PCR产物与人OCT 4基因的同源性为99.17%。结论：OCT 4在角膜上皮基底层表达。
Purpose: Transcription factors act as molecular switches to activate or repress specific genes, which in turn control cellular phenotype. Transcription factor OCT4 is critical for self-renewal and maintenance of embryonic stem cells. OCT4 expression is down-regulated in all differentiated somatic cell types in vitro and in vivo. Our aim is to investigate whether the human corneal epithelium expresses OCT4. Materials and Methods: OCT4 protein was examined in nine human cornea specimens and in human testicular seminoma positive control samples by fluorescence immunohistochemistry. OCT4 mRNA was studied in human corneal epithelial as well as human embryonic stem cells (ESC). The RT-PCR products were further analyzed by DNA sequencing. Results: All of the corneas displayed nuclear staining patterns, limited to the corneal epithelial basal layer. The OCT4 positive staining cells resided mainly in the limbal epithelial basal layer, but some were also found in the peri-limbal and central corneal epithelial basal layers. OCT4 mRNA was detected in the epithelium of all human corneas and human ESC cells. Neither OCT4 mRNA nor protein was detected from negative controls. The sequencing analysis showed that the base sequences of PCR products were 99.17% similar to the human OCT4 gene. Conclusion: The basal layer of corneal epithelium expresses OCT4.