Arabidopsis GCN5, HD1, and TAF1/HAF2 interact to regulate histone acetylation required for light-responsive gene expression
Arabidopsis GCN5, HD1, and TAF1/HAF2 interact to regulate histone acetylation required for light-responsive gene expression
复制标题
DOI：
10.1105/tpc.106.043489
复制
发表时间：
2006-11-01
期刊：
影响因子：
11.6
通讯作者：
Zhou, Dao-Xiu
中科院分区：
文献类型：
--
作者：
Benhamed, Moussa;Bertrand, Claire;Zhou, Dao-Xiu
我们先前的研究表明，组蛋白乙酰转移酶TAF1/HAF2是光调控植物生长和基因表达所必需的，我们还发现组蛋白乙酰转移酶GCN5和组蛋白去乙酰化酶HD1/HDA19也参与了这一调控。GCN5突变导致长下胚轴表型，光诱导基因表达减少，而HD1突变则产生相反的效应。双突变体gcn5hd1恢复了正常的光形态发生表型。相反，双突变体gcn5 TAF1导致光调控基因表达的进一步丧失。Gcn5减少了组蛋白H3和H4的乙酰化，主要在核心启动子区域，而HD1增加了核心和更上游启动子区域的乙酰化。目的启动子上的H3K9、H3K27和H4K12乙酰化均需要GCN5和TAF1，而H3K14乙酰化仅依赖于GCN5。有趣的是，gcn5 TAF1主要对H3K9乙酰化有累积作用。另一方面，HD1诱导H3K9、H3K27、H4K5和H4K8上的乙酰化增加。GCN5也被证明与光响应启动子直接相关。这些结果表明，受GCN5、TAF1和HD1调控的特定组蛋白赖氨酸残基的乙酰化是光调控基因表达所必需的。
We previously showed that Arabidopsis thaliana histone acetyltransferase TAF1/HAF2 is required for the light regulation of growth and gene expression, and we show here that histone acetyltransferase GCN5 and histone deacetylase HD1/HDA19 are also involved in such regulation. Mutation of GCN5 resulted in a long-hypocotyl phenotype and reduced light-inducible gene expression, whereas mutation of HD1 induced opposite effects. The double mutant gcn5 hd1 restored a normal photomorphogenic phenotype. By contrast, the double mutant gcn5 taf1 resulted in further loss of light-regulated gene expression. gcn5 reduced acetylation of histones H3 and H4, mostly on the core promoter regions, whereas hd1 increased acetylation on both core and more upstream promoter regions. GCN5 and TAF1 were both required for H3K9, H3K27, and H4K12 acetylation on the target promoters, but H3K14 acetylation was dependent only on GCN5. Interestingly, gcn5 taf1 had a cumulative effect mainly on H3K9 acetylation. On the other hand, hd1 induced increased acetylation on H3K9, H3K27, H4K5, and H4K8. GCN5 was also shown to be directly associated with the light-responsive promoters. These results suggest that acetylation of specific histone Lys residues, regulated by GCN5, TAF1, and HD1, is required for light-regulated gene expression.