Maize host requirements for Ustilago maydis tumor induction.
Maize host requirements for Ustilago maydis tumor induction.
复制标题
玉米黑粉菌肿瘤诱导的玉米宿主要求。
DOI：
10.1007/s00497-009-0109-0
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发表时间：
2010
影响因子：
--
通讯作者：
Skibbe,DavidS
中科院分区：
文献类型：
--
作者：
Walbot,Virginia;Skibbe,DavidS
活体营养型病原菌黑粉菌可能通过改变玉米营养体和花的发育方向而引起肿瘤。真菌注射到未成熟的雄穗后，在所有花器官中发现肿瘤，器官易感性的进展反映了细胞分裂灶在发育中的小穗中的顺序位置。肿瘤形成区和正常小穗成熟和花粉脱落的区域之间有明显的分界线;在肿瘤区内和紧邻肿瘤区，发育中的花药通常早熟出现，并表现出一系列发育缺陷，这表明U.maydissignals和宿主反应在空间上受到限制。玉米雄性不育突变体的花药细胞分裂模式和细胞命运收购的缺陷减数分裂前形成正常的成年叶片肿瘤，但未能形成花药肿瘤。茉莉酸甲酯和油菜素类固醇phenocopied这些早期作用花药发育突变体产生不育区内的雄穗，从来没有形成肿瘤。虽然生长素、细胞分裂素、脱落酸和赤霉素都不能阻止雄穗的发育，但赤霉素信号缺陷的Dwarf 8突变体没有雄穗瘤;赤霉素含量减少的花药1突变体形成正常瘤;而叶组织过度生长的Knotted 1形成更大的营养体和雄穗瘤。我们提出了宿主生长潜力和组织特性调节U. maydis重新定向分化和诱导肿瘤的能力的假设。
The biotrophic pathogenUstilago maydiscauses tumors by redirecting vegetative and floral development in maize (Zea maysL.). After fungal injection into immature tassels, tumors were found in all floral organs, with a progression of organ susceptibility that mirrors the sequential location of foci of cell division in developing spikelets. There is sharp demarcation between tumor-forming zones and areas with normal spikelet maturation and pollen shed; within and immediately adjacent to the tumor zone, developing anthers often emerge precociously and exhibit a range of developmental defects suggesting thatU.maydissignals and host responses are restricted spatially. Male-sterile maize mutants with defects in anther cell division patterns and cell fate acquisition prior to meiosis formed normal adult leaf tumors, but failed to form anther tumors. Methyl jasmonate and brassinosteroid phenocopied these early-acting anther developmental mutants by generating sterile zones within tassels that never formed tumors. Although auxin, cytokinin, abscisic acid and gibberellin did not impede tassel development, theDwarf8mutant defective in gibberellin signaling lacked tassel tumors; theanther ear1mutant reduced in gibberellin content formed normal tumors; andKnotted1, in which there is excessive growth of leaf tissue, formed much larger vegetative and tassel tumors. We propose the hypothesis that host growth potential and tissue identity modulate the ability ofU.maydisto redirect differentiation and induce tumors.