CHRONIC SOCIAL DEFEAT STRESS INCREASES DOPAMINE D2 RECEPTOR DIMERIZATION IN THE PREFRONTAL CORTEX OF ADULT MICE
CHRONIC SOCIAL DEFEAT STRESS INCREASES DOPAMINE D2 RECEPTOR DIMERIZATION IN THE PREFRONTAL CORTEX OF ADULT MICE
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DOI：
10.1016/j.neuroscience.2015.10.024
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发表时间：
2015-12-17
期刊：
影响因子：
3.3
通讯作者：
Chung, Y. -C.
中科院分区：
文献类型：
--
作者：
Bagalkot, T. R.;Jin, H. -M.;Chung, Y. -C.
本研究旨在探讨慢性社会失败应激对多巴胺受体和参与内吞后转运途径的蛋白质的影响。在社交失败应激10天后，将成年小鼠分为易感组和非易感组。Western印迹分析用于测量多巴胺D2受体（D2 R）、短形式（D2 S）和长形式（D2 L）以及D2 R单体和二聚体、多巴胺D1受体（D1 R）、神经元钙传感器-1（NCS-1）和G蛋白偶联受体相关分选蛋白-1（GASP-1）的蛋白质表达水平，采用逆转录-聚合酶链反应（RT-PCR）检测D2 S、D2 L、D2 R单体和二聚体以及D1 Rs在不同脑区的mRNA表达水平。我们观察到增加的D2 S，D2 L和D2 Rs二聚体的表达在前额叶皮层（PFC）的易感和/或不易感小鼠与对照组相比。关于mRNA表达水平的唯一显著发现是与对照组相比，易感和不易感小鼠杏仁核（AMYG）中D2 S mRNA的表达较低。本研究表明，慢性社会失败应激诱导增加D2 S，D2 L和D2 R二聚体的表达在易感和/或不易感小鼠的PFC。(C)2015年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The present study aimed to examine the effects of chronic social defeat stress on the dopamine receptors and proteins involved in post-endocytic trafficking pathways. Adult mice were divided into susceptible and unsusceptible groups after 10 days of social defeat stress. Western blot analysis was used to measure the protein expression levels of dopamine D2 receptors (D2Rs), a short (D2S) and a long form (D2L) and, D2R monomers and dimers, dopamine D1 receptors (D1Rs), neuronal calcium sensor-1 (NCS-1) and G protein-coupled receptor-associated sorting protein-1 (GASP-1), and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the mRNA expression levels of D2S, D2L, D2R monomers and dimers, and D1Rs in different brain areas. We observed increased expression of D2S, D2L and D2Rs dimers in the prefrontal cortex (PFC) of susceptible and/or unsusceptible mice compared with controls. The only significant findings with regard to mRNA expression levels were lower expression of D2S mRNA in the amygdala (AMYG) of susceptible and unsusceptible mice compared with controls. The present study demonstrated that chronic social defeat stress induced increased expression of D2S, D2L, and D2R dimers in the PFC of susceptible and/or unsusceptible mice. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.