A TIME SEQUENCE STUDY OF FAT ABSORPTION IN RAT JEJUNUM
A TIME SEQUENCE STUDY OF FAT ABSORPTION IN RAT JEJUNUM
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DOI：
10.1002/aja.1001180108
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发表时间：
1966-01-01
影响因子：
--
通讯作者：
JERSILD, RA
中科院分区：
文献类型：
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作者：
JERSILD, RA
用电镜和放射自显影技术观察大鼠小肠上皮细胞对脂肪的吸收和转运。生理脂肪食糜，从脂肪喂养的供体大鼠制备，注射到结扎段的禁食受体动物的空肠上段。从1到30分钟的时间序列中取出组织样品。将油酸-H3加入食糜中进行光镜和电镜放射自显影。结果表明，脂肪通过上皮吸收和转运非常迅速。1分钟内，在内质网和高尔基体中可以看到脂肪滴。通过放射自显影识别整个细胞中脂肪滴附近的标记，表明脂肪在实验期间而不是之前被吸收。高尔基体内脂肪积累的证据出现在1和2分钟之间。这种积累可能是吸收的限速步骤的指示。有人建议，高尔基体内发生的现象是部分负责与脂肪，这是明显的，在8至10分钟的外观细胞饱和。顺序模式可以遵循细胞沿着绒毛的整个长度。
Fat absorption and transport in rat intestinal epithelial cells were followed by electron microscopy and radioautography. Physiological fatty-chyme, prepared from fat-fed donor rats, was injected into ligated segments of the upper jejunum of fasting recipient animals. Samples of tissue were removed in a time sequence from 1 to 30 min. Oleic acid-H3 was added to the chyme for light and electron microscopic radioautography. The results indicate that fat is absorbed and transported through the epithelium very rapidly. Within 1 min., fat droplets can be seen in the endoplasmic reticulum and the Golgi apparatus. Fat is shown to be absorbed during, not prior to, experimentation by the radioautographic identification of labeling in the vicinity of fat droplets throughout the cell. Evidence of accumulation of fat within the Golgi apparatus appears between 1 and 2 min. Such accumulation may be indicative of a rate-limiting step in absorption. It is suggested that phenomena occurring withing the Golgi apparatus are in part responsible for the appearance of cellular saturation with fat, which is evident in 8 to 10 min. The sequential pattern can be followed to cells along the entire length of the villus.