Characterization of NtSTOP1-regulating genes in tobacco under aluminum stress
Characterization of NtSTOP1-regulating genes in tobacco under aluminum stress
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DOI：
10.1080/00380768.2019.1603064
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发表时间：
2019-04-15
影响因子：
2
通讯作者：
Koyama, Hiroyuki
中科院分区：
文献类型：
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作者：
Ito, Hiroki;Kobayashi, Yuriko;Koyama, Hiroyuki
锌指转录因子STOP 1-like蛋白在陆地植物中是一种保守的转录因子，控制着植物耐铝基因的表达。我们在烟草（烟草）中进行转录组分析，以确定NtSTOP 1调节铝耐性基因，其表达受到其STOP 1样蛋白的调控，然后将它们与拟南芥（拟南芥）和水稻（水稻）中的基因进行比较。市售的寡核苷酸微阵列鉴定了NtSTOP 1-RNAi烟草系中的96个基因，其在Al胁迫下显示出比野生型更低的表达水平。结果表明，大多数NtSTOP 1控制的基因是共同的拟南芥和水稻，虽然有些是在拟南芥，这是更敏感的铝比烟草和水稻中丢失。例如，NtSTOP 1调节水稻STAR 1的直系同源物的表达，其表达在水稻中由STOP 1样蛋白ART 1调节，但在拟南芥中不受调节。NtSTOP 1-RNAi株系中参与氮代谢的基因表达水平较低，包括拟南芥中不受STOP 1调控的其他基因。此外，NtSTOP 1控制发酵途径酶的几个基因的表达，尽管在拟南芥中尚未发现。我们还揭示了NtSTOP 1的结合位点包含的GGNVS基序的NtMATE启动子。导致STOP 1/NtSTOP 1分别与AtALMT 1/NtMATE结合的基序是相似的，但AtALMT 1和NtMATE是根本不同的基因。烟草和拟南芥之间STOP 1调节系统的这种差异与几个靶基因中顺式元件突变的差异有关。
Zinc finger transcription factors, STOP1-like proteins, are conserved among land plant species and control the expression of aluminum tolerance genes in various plant species. We performed transcriptome analyses in tobacco (Nicotiana tabacum) to identify NtSTOP1-regulating Al tolerance genes, the expression of which was regulated by their STOP1-like proteins, and then compared them with those in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa). A commercially available oligo microarray identified 96 genes in a NtSTOP1-RNAi tobacco line that showed lower expression levels than in the wild type under Al stress. It was shown that most NtSTOP1 controlled genes were common to both Arabidopsis and rice, although some were missing in Arabidopsis, which is more sensitive to Al than tobacco and rice. For example, NtSTOP1 regulated the expression of orthologs of rice STAR1, the expression of which was regulated by the STOP1-like protein ART1 in rice, but not in Arabidopsis. The NtSTOP1-RNAi line showed lower expression levels of genes involved in N-metabolism, including additional genes which were not regulated by STOP1 in Arabidopsis. In addition, NtSTOP1 controlled the expression of several genes for enzymes of fermentation pathways, although it was not identified in Arabidopsis. We also revealed the NtSTOP1 binding site containing the GGNVS motif of the NtMATE promoter. The motif that causes STOP1/NtSTOP1 to bind to AtALMT1/NtMATE, respectively, was similar, but AtALMT1 and NtMATE are fundamentally different genes. This difference in the STOP1-regulating system between tobacco and Arabidopsis is linked to differences in mutations in cis-elements in several target genes.