Regeneration of the Trachea Using a Bioengineered Scaffold with Adipose-Derived Stem Cells
Regeneration of the Trachea Using a Bioengineered Scaffold with Adipose-Derived Stem Cells
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DOI：
10.1177/000348940811700609
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发表时间：
2008-06
期刊：
影响因子：
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通讯作者：
Teruhisa Suzuki;Ken Kobayashi;Y. Tada;Yukie Suzuki;I. Wada;Tatuo Nakamura;K. Omori
中科院分区：
文献类型：
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作者：
Teruhisa Suzuki;Ken Kobayashi;Y. Tada;Yukie Suzuki;I. Wada;Tatuo Nakamura;K. Omori
目的：本课题组研制了一种以胶原海绵为支架的人工气管组织再生支架，并已应用于临床。然而，人工移植物需要约2个月的上皮再生。本研究的目的是通过生物工程支架的有效使用来加速气管的再生过程。使用具有多向分化能力的脂肪源性干细胞（ASC）。在我们的研究中，我们植入了一个生物工程支架，包括自体ASC到大鼠气管缺损。研究方法：胶原凝胶，包括标记有单体黄色荧光蛋白的ASC，被层压到胶原海绵的表面上以形成生物工程支架。将该支架植入大鼠气管缺损处。还植入了不含ASC的对照支架。结果如下：在植入后第14天，在接受具有包括ASC的生物工程支架的植入物的大鼠中观察到具有分化良好的纤毛细胞和杯状细胞以及新血管形成的假复层柱状上皮。结论：这些结果表明植入的ASC加速了再生气管的新血管形成和上皮化。因此，我们新开发的生物工程支架有助于气管再生。
Objectives: Our group has developed and clinically applied an artificial graft made from a collagen sponge scaffold for the regeneration of tracheal tissue. However, the artificial graft requires about 2 months for epithelial regeneration. The purpose of the present study was to accelerate the regeneration process of the trachea through the effective use of a bioengineered scaffold. Adipose-derived stem cells (ASCs) with multilineage differentiation capability were used. In our study, we implanted a bioengineered scaffold that included autologous ASCs into tracheal defects in rats. Methods: Collagen gel, including ASCs labeled with monomeric yellow fluorescent protein, was layered onto the surface of the collagen sponge to form a bioengineered scaffold. This scaffold was implanted into the tracheal defects in rats. A control scaffold without ASCs was also implanted. Results: On day 14 after implantation, a pseudostratified columnar epithelium with well-differentiated ciliated and goblet cells and neovascularization was observed in rats that received the implant with the bioengineered scaffold that included ASCs. Conclusions: These results suggested that implanted ASCs accelerated neovascularization and epithelialization on the regenerated trachea. Thus, our newly developed bioengineered scaffold contributes to tracheal regeneration.