Adherence characteristics to human small intestinal mucosa of Escherichia coli isolated from patients with diarrhea or urinary tract infections.
Adherence characteristics to human small intestinal mucosa of Escherichia coli isolated from patients with diarrhea or urinary tract infections.
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从腹泻或尿路感染患者中分离出的大肠杆菌对人小肠粘膜的粘附特征。
DOI：
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发表时间：
1990
影响因子：
6.4
通讯作者：
Takeshi Yokota
中科院分区：
文献类型：
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作者：
Tatsuo Yamamoto;Kazuhiko Fujita;Takeshi Yokota
使用福尔马林固定的人回肠粘膜和福尔马林固定的或未处理的（天然）人膀胱粘膜来测试大肠杆菌肠致病性（ETEC）或尿路致病性（UPEC）对人的粘附能力。在37 ℃条件下，ETEC在CFA/I或CFA/II皮利培养基上生长3 h后，具有典型的周毛鞭毛，并能与人回肠淋巴滤泡和绒毛上皮牢固粘附。与之相反，E.具有CFA/I或CFA/II皮利且具有非常弱或无运动性的大肠杆菌细胞显示出低水平的对上皮的粘附。UPEC具有1型皮利，很少有鞭毛，与人膀胱粘膜有很强的粘附，但不与人回肠上皮粘附。1型具菌毛E.大肠杆菌分离的人粪便中的行为一样UPEC。此外，存在于人回肠淋巴滤泡上皮中的M细胞（微折叠）为1型皮利提供粘附位点，但不为CFA/I或CFA/II皮利提供粘附位点。这些数据证明了细菌运动性在体外有效粘附于人回肠上皮中的重要性，与人尿上皮相反，以及细菌粘附于M细胞微折叠的粘附素特异性。
Formalin-fixed human ileal mucosa and formalin-fixed or untreated (native) human urinary bladder mucosa were used to test the adherence ability of Escherichia coli enterotoxigenic (ETEC) or uropathogenic (UPEC) for humans. When grown on colonization factor antigen (CFA) agar for 3 h at 37 degrees C, ETEC with CFA/I or CFA/II pili had typical peritrichous flagella and adhered strongly to human ileal lymphoid follicle and villus epithelium. In contrast, E. coli cells with CFA/I or CFA/II pili and possessing very weak or no motility displayed low levels of adherence to the epithelium. UPEC, which possessed type 1 pili and rarely had flagella, strongly adhered to human urinary bladder mucosa but not to human ileal epithelium. Type 1 pili-possessing E. coli isolated from human feces behaved as did UPEC. Moreover, M cells (microfolds) present in human ileal lymphoid follicle epithelium provided adherence sites for type 1 pili but not for CFA/I or CFA/II pili. These data demonstrate the importance of bacterial motility in efficient in vitro adherence to human ileal epithelia, in contrast to human urinary epithelia, and the adhesin specificity of bacterial adherence to M cell microfolds.