Effects of varying gibberellin and abscisic acid levels on embryo development and endogenous hormones in recalcitrant Panax notoginseng seeds during the after-ripening process
Effects of varying gibberellin and abscisic acid levels on embryo development and endogenous hormones in recalcitrant Panax notoginseng seeds during the after-ripening process
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DOI：
10.19675/j.cnki.1006-687x.2019.08033
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发表时间：
2020-01-01
影响因子：
--
通讯作者：
Chen Junwen
中科院分区：
文献类型：
--
作者：
Ge Na;Yang Ling;Chen Junwen
了解三七抗性种子休眠和萌发的机制。陈富华，种子用外源激素赤霉素（GA3）和脱落酸（ABA）处理。然后观察胚胎发育，并分析成熟后6种内源激素水平。结果表明，胚与胚乳之比在后熟过程中逐渐增大。外源激素GA(3)浓度为500 mg/L时，萌发率显著提高，ABA浓度为10 mg/L时，萌发率显著降低。内源激素ABA和GA(3)的浓度分别达到2.83 ng/g和27.85 ng/g。处理后的种子在成熟初期浓度显著高于对照组，在成熟过程中逐渐降低。后熟过程中IAA浓度也逐渐降低，但两组间差异不显著。在此过程中，水杨酸（SA）的浓度变化不大。茉莉酸甲酯（Methyl jasmonate， JA）在对照组和ABA处理组均逐渐升高，ABA处理组在成熟后50 d达到最高值1.25 ng/g。而GA(3)治疗组JA先升高后降低。转zeicin （tZT）浓度在对照组基本不变，GA(3)处理组逐渐升高，ABA处理组逐渐降低。结果表明，外源植物激素的施用可以有效调节内源植物激素，从而间接影响三七种子的后熟过程和萌发。此外，高GA(3)浓度可以打破休眠，缩短三七种子的后熟过程，而低ABA浓度可以显著延长三七种子的休眠时间。
To understand the mechanisms behind the dormancy and germination of recalcitrant seed from Panax notoginseng (Burk.) F. H. Chen, seeds were treated by exogenous hormone gibberellin (GA3) and abscisic acid (ABA). Embryo development was then observed and six endogenous hormone levels were analyzed during the after-ripening process. The results showed that the ratio of embryos to endosperm increases gradually during the after-ripening process. Germination rate significantly increased when seeds were treated with exogenous hormone GA(3) at a concentration of 500 mg/L, and it was significantly inhibited with treatment by ABA at a concentration of 10 mg/L. The concentration of endogenous hormones ABA and GA(3) reached 2.83 ng/g and 27.85 ng/g, respectively. These concentrations were significantly higher in the treated seeds than in the control groups at the initial after-ripening stage and the two hormones gradually decreased during the after-ripening process. The IAA concentration also gradually decreased during the after-ripening process, but there was no significant difference between the two groups. The concentration of salicylic acid (SA) varied little during the process. Methyl jasmonate (JA) gradually increased in both the control groups and the ABA treated groups, with the maximum value reaching 1.25 ng/g in the ABA treatment group at 50 d of the after-ripening process. However, in the GA(3) treatment group, JA initially increased and then decreased. The concentration of transzeicin (tZT) was generally unchanged in the control group, but gradually increased in the GA(3) treatment group and gradually decreased in the ABA treatment group. Our results indicate that the application of exogenous phytohormones may effectively regulate endogenous phytohormones, thereby indirectly influencing the afterripening process and germination of P. notoginseng seeds. In addition, high GA(3) concentration may break dormancy and shorten the after-ripening process, while low ABA concentration may significantly prolong the dormancy of P. notoginseng seeds.