Adult hippocampal cell proliferation is suppressed with estrogen withdrawal after a hormone-simulated pregnancy
Adult hippocampal cell proliferation is suppressed with estrogen withdrawal after a hormone-simulated pregnancy
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DOI：
10.1016/j.yhbeh.2008.02.023
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发表时间：
2008-06-01
影响因子：
3.5
通讯作者：
Galea, Liisa A. M.
中科院分区：
文献类型：
--
作者：
Green, Amanda D.;Galea, Liisa A. M.
怀孕后的Escherichia coli撤退被假设为沉淀抑郁症状在脆弱的妇女。在雌性大鼠中诱导了一种模拟妊娠的雌激素，并研究了“产后”雌二醇下降对海马细胞增殖的影响。所有组在治疗前均切除卵巢或进行假手术。将大鼠随机分为“产后”、“产后”+ EB（苯甲酸雌二醇）、“产后”+ DPN（二芳基丙腈; ER β激动剂）、“产后”+ IMI（丙咪嗪;三环类抗抑郁药）、假手术、卵巢切除（OVX）、假手术+ IMI或OVX + IMI组。所有“产后”组在23天内接受激素注射（雌二醇和孕酮）以模拟怀孕，而IMI组也每天接受丙咪嗪注射。第23天后，“产后”大鼠从模拟妊娠的子宫中退出（模拟产后性腺激素下降），而其他“产后”治疗组每天接受DPN，EB或IMI注射。在“产后”第3天，向所有大鼠注射溴脱氧尿苷（BrdU; DNA合成标记物），并在24小时后灌注，以评估齿状回中的细胞增殖和细胞死亡。仅在“产后”和“产后”+ EB组中，“产后”激素戒断降低了海马细胞增殖。慢性丙咪嗪显着增加海马细胞增殖假+ IMI，但不OVX + IMI大鼠表明丙咪嗪的影响，以增加海马细胞增殖雌性大鼠的生殖状态有关。细胞死亡（固缩细胞）仅在“产后”组中减少。总之，这些结果表明，性腺激素在伴随产后抑郁症模型的细胞变化中起着重要但复杂的作用。(C)2008年爱思唯尔公司All rights reserved.
Estradiol withdrawal after pregnancy is hypothesized to precipitate depressive symptoms in vulnerable women. A hormone-simulated pregnancy was induced in female rats and the effects of a 'postpartum' drop in estradiol on hippocampal cell proliferation were examined. All groups were ovariectomized or given sham surgery prior to treatment. Rats were randomly assigned to 'postpartum', 'postpartum' + EB (estradiol benzoate), 'postpartum' + DPN (diarylpropionitrile; an ER beta agonist),'postpartum' + IMI (imipramine; a tricyclic antidepressant), sham, ovariectomized (OVX), sham + IMI or OVX + IMI groups. All 'postpartum' groups received hormone injections (estradiol and progesterone) over 23 days to simulate pregnancy, while IMI groups also received daily imipramine injections. After day 23, 'postpartum' rats were withdrawn from the hormone-simulated pregnancy (mimicking the postpartum drop in gonadal hormones), while other 'postpartum' treatment groups received daily injections of DPN, EB or IMI. On day 3 'postpartum' all rats were injected with bromodeoxyuridine (BrdU; a DNA synthesis marker) and perfused 24 h later to assess cell proliferation and cell death in the dentate gyrus. 'Postpartum' hormone withdrawal decreased hippocampal cell proliferation in the 'postpartum' and 'postpartum' + EB groups only. Chronic imipramine significantly increased hippocampal cell proliferation in sham + IMI, but not OVX + IMI rats suggesting that imipramine's effects to increase hippocampal cell proliferation in female rats is related to reproductive status. Cell death (pyknotic cells) was decreased only in the 'postpartum' group. Together, these results suggest an important, though complex, role for gonadal hormones in the cellular changes accompanying this model of postpartum depression. (C) 2008 Elsevier Inc. All rights reserved.