Insertion of the bacterial gpt gene into the germ line of mice by retroviral infection.
Insertion of the bacterial gpt gene into the germ line of mice by retroviral infection.
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通过逆转录病毒感染将细菌 gpt 基因插入小鼠种系中。
DOI：
10.1073/pnas.82.20.6927
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发表时间：
1985
影响因子：
11.1
通讯作者：
Jaenisch,R
中科院分区：
文献类型：
--
作者：
Jähner,D;Haase,K;Mulligan,R;Jaenisch,R
在单个基因座上遗传传递外源性Moloney小鼠白血病病毒（Mo-MuLV）的小鼠亚株已经通过感染着床前胚胎获得。在这里，我们探索逆转录病毒载体将非病毒基因转移到小鼠种系的潜力。植入前小鼠胚胎与一种产生重组逆转录病毒的细胞系共培养，该病毒的基因组携带大肠杆菌基因gpt。我们发现，载体序列以与mo - mulv辅助序列相似的频率插入胚胎基因组和种系。一种新的小鼠菌株Mgpt-1被开发出来，它是单一MSVgpt前病毒基因组的纯合子。前病毒序列高度甲基化，在Mgpt-1小鼠的组织中不表达。当来源于转基因动物的细胞用5-氮杂胞苷处理时，前病毒序列没有甲基化，而是转录激活。这些结果表明，在病毒长末端重复序列控制下的非病毒基因在转移到动物种系时失活。
Mouse substrains genetically transmitting the exogenous Moloney murine leukemia virus (Mo-MuLV) at a single locus have been derived previously by infection of preimplantation embryos. Here we explore the potential of retroviral vectors for transferring nonviral genes into the germ line of mice. Preimplantation mouse embryos were cocultivated with a cell line that produces a recombinant retrovirus whose genome carries the Escherichia coli gene gpt. We show that the vector sequence was inserted into the genome of the embryo and into the germ line at a frequency similar to that for the Mo-MuLV-helper sequence. A new mouse strain, Mgpt-1, was developed that is homozygous for a single MSVgpt proviral genome. The proviral sequences were highly methylated and not expressed in tissues of Mgpt-1 mice. When cells derived from transgeneic animals were treated with 5-azacytidine, the proviral sequences were not methylated and were transcriptionally activated. These results indicate that nonviral genes that are under the control of the viral long terminal repeat are inactivated when transferred into the germ line of animals.