Three-dimensional organization of the connective tissue fibers of the human pancreas: a scanning electron microscopic study of NaOH treated-tissues.
Three-dimensional organization of the connective tissue fibers of the human pancreas: a scanning electron microscopic study of NaOH treated-tissues.
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人类胰腺结缔组织纤维的三维组织：NaOH 处理组织的扫描电子显微镜研究。
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
O. Ohtani
中科院分区：
文献类型：
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作者：
O. Ohtani
本文描述了一种用扫描电子显微镜（SEM）研究网状纤维原始形状和位置的方法。该技术被用来展示人类胰腺网状纤维的三维组织。用10%的NaOH水溶液在室温下处理3-4天，细胞元素被有效去除。围绕腺泡和导管的网状纤维薄层形成了三维间质室。血管的网状纤维鞘穿过隔室。在小叶中，散有朗格汉斯岛的圆形或椭圆形蒴果。被膜也由网状纤维组成。在囊内，用于容纳胰岛毛细血管的网状纤维鞘，代表毛细血管周围空间，形成三维吻合网络。胰岛内被网状纤维包裹的毛细血管的通道与周围外分泌胰腺的通道是连续的；因此，胰岛素-腺泡门静脉系统被证实存在于人类胰腺中。本研究还认为，本方法对胰岛微血管组织的检查是有用的。
A method for scanning electron microscope (SEM) study of reticular fibers in their original shapes and locations is described. This technique was employed to demonstrate the three-dimensional organization of the reticular fibers of the human pancreas. The cellular elements were effectively removed by treatment of the tissue pieces with a 10% aqueous solution of NaOH for 3-4 days at room temperature. Thin layers of the reticular fibers surrounding the acini and ducts formed a three-dimensional interstitial compartment. The reticular fiber sheaths for the blood vessels coursed through the compartment. In the lobule, there were scattered round or oval capsules for the islets of Langerhans. The capsule also consisted of reticular fibers. Within the capsule, reticular fiber sheaths for accommodating islet capillaries, representing the pericapillary spaces, formed a three-dimensionally anastomosing network. The channels for the capillaries ensheathed by the reticular fibers in the islet were continuous with those in the surrounding exocrine pancreas; thus, the insulo-acinar portal system was confirmed to exist in the human pancreas. This study also maintains that the present method is useful for examining the microvascular organization of the islet.