Identification and risk assessment of spinosad resistance in a California population of Drosophila suzukii
Identification and risk assessment of spinosad resistance in a California population of Drosophila suzukii
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DOI：
10.1002/ps.5240
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发表时间：
2019-05-01
影响因子：
4.1
通讯作者：
Zalom, Frank G.
中科院分区：
文献类型：
--
作者：
Gress, Brian E.;Zalom, Frank G.
背景生物杀虫剂多杀菌素是用于管理点翅果蝇（Drosophila suzukii（Matsumura））的最广泛使用的杀虫剂之一，并且对于防止有机浆果生产中的果实侵染至关重要。然而，最近的报道引起了人们的担忧，即这种材料的功效在加利福尼亚州沃森维尔附近的田地里正在下降，沃森维尔是美国商业浆果生产的主要中心，也是D.铃木病在北美有报道。结果在本研究中，我们对D.使用广泛实施的生物测定方案，从沃森维尔附近的商业树莓种植地以及加州的第二个未处理的地点获得Suzukii。我们发现，沃森维尔苍蝇表现出多杀菌素LC 50值比未处理的位置高4.3-7.7倍，比以前报道的敏感基线高11.6-22.4倍。此外，多杀菌素的耐受性继续增加后，额外的选择五代，虽然这一结果是只有在长期暴露于残留物的统计学显着。结论这些研究结果证实，多杀菌素耐药性正在出现在沃森维尔地区和文件的第一个已知的发生在美国，提出了迫切需要的替代管理策略，以控制这种害虫的发展。需要进一步的工作来解决赋予多杀菌素抗性的潜在分子机制。并评估这种特性传播到新种群中的潜力。(c)2018年化学工业协会
Background The bioinsecticide spinosad is among the most widely used insecticides for managing spotted-wing drosophila, Drosophila suzukii (Matsumura), and is critical for preventing fruit infestation in organic berry production. Recent reports, however, have raised concerns that the efficacy of this material is declining in fields near Watsonville, CA, a major hub of commercial berry production in the USA and the first location where D. suzukii was reported in North America. Results In this study, we performed dose-response analyses on D. suzukii from commercial raspberry plantings near Watsonville as well as a second untreated site in California using a widely implemented bioassay protocol. We found that Watsonville flies exhibited spinosad LC50 values 4.3-7.7 times higher than those from the untreated location and 11.6-22.4 times higher than previously reported susceptible baselines. Additionally, tolerance to spinosad continued to increase after additional selection for five generations, though this result was only statistically significant after prolonged exposure to residues. Conclusions These findings confirm that spinosad resistance is emerging in the Watsonville area and document the first known occurrence in the USA, presenting an urgent need for the development of alternative management strategies to control this pest. Additional work is needed to resolve the underlying molecular mechanism(s) that confers spinosad resistance in D. suzukii and assess the potential for this trait to spread into new populations. (c) 2018 Society of Chemical Industry