NEUTRALIZATION OF THE AIDS RETROVIRUS BY ANTIBODIES TO A RECOMBINANT ENVELOPE GLYCOPROTEIN
NEUTRALIZATION OF THE AIDS RETROVIRUS BY ANTIBODIES TO A RECOMBINANT ENVELOPE GLYCOPROTEIN
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DOI：
10.1126/science.3014647
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发表时间：
1986-07-11
期刊：
影响因子：
56.9
通讯作者：
BERMAN, PW
中科院分区：
文献类型：
--
作者：
LASKY, LA;GROOPMAN, JE;BERMAN, PW
哺乳动物细胞系已被改造以产生分泌形式的AIDS逆转录病毒包膜糖蛋白。重组蛋白已从生长条件培养基中分离出来，并用于免疫动物。发现针对重组分子的抗体与病毒感染细胞中产生的包膜糖蛋白反应。此外，这些抗体能够在体外中和测定中直接抑制AIDS逆转录病毒。这里报道的表达系统应提供足够数量的艾滋病逆转录病毒包膜蛋白的生物学和疫苗接种研究。
Mammalian cell lines have been engineered to produce a secreted form of the AIDS retrovirus envelope glycoprotein. The recombinant protein has been isolated from growth-conditioned culture media and used to immunize animals. Antibodies directed against the recombinant molecule were found to react with the envelope glycoprotein produced in virus-infected cells. Furthermore, these antibodies were able to directly inactivate the AIDS retrovirus in a neutralization assay in vitro. The expression system reported here should provide sufficient quantities of the AIDS retrovirus envelope protein for biological and vaccination studies.