Streptomyces otsuchiensis sp. nov., a biosurfactant-producing actinobacterium isolated from marine sediment
Streptomyces otsuchiensis sp. nov., a biosurfactant-producing actinobacterium isolated from marine sediment
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奥苏链霉菌 (Streptomyces otsuchiensis)
DOI：
10.1099/ijsem.0.003638
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发表时间：
2019
影响因子：
2.8
通讯作者：
Tomohiko Tamura
中科院分区：
文献类型：
--
作者：
Takeshi Terahara;Takuya Naemura;Yukiko Nampo;Takeshi Kobayashi;Chiaki Imada;Moriyuki Hamada;Tomohiko Tamura
从日本岩手县大池湾海洋沉积物中分离到一株产表面活性剂的新型放线菌OTB305T，并采用多相法对其分类位置进行了研究。基于16S rRNA基因序列的系统发育分析表明，菌株otb305t与bohaiensis streptomyces jcm 19630T（98.8%）和lonarensisstreptomyces dsm 42084T（98.8%）亲缘关系较近。菌株otb305t的化学分类特征与链霉菌属一致：细胞壁肽聚糖的二氨基为二氨基苯甲酸；全细胞水解物含有葡萄糖，缺乏特有的主要糖；主要的类异戊二烯醌是MK-9（H8）和MK-9(H6)；极性脂质为磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇和一种未识别的磷脂；细胞脂肪酸主要为iso-C16: 0、C16: 0和C16: 1ω7c；基因组DNA G+C含量为72.83 mol%。然而，基于平均核苷酸身份和一些表型特征的基因组相关性分析显示，菌株otb305t与近亲链霉菌物种有所区别。因此，菌株otb305代表了链霉菌属的一个新种，因此被命名为链霉菌。11月被提议。型应变为OTB305T（=NBRC 113255T=TBRC 9682T）。
A novel actinobacterium producing biosurfactant, designated OTB305T, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305Twas closely related toStreptomyces bohaiensisJCM 19630T(98.8 %) andStreptomyces lonarensisDSM 42084T(98.8 %). The chemotaxonomic characteristics of strain OTB305Tcorresponded to those of the genusStreptomycesas follows: the diamino acid of the cell-wall peptidoglycan wasll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H8) and MK-9(H6); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C16 : 0, C16 : 0and C16 : 1ω7c; and the genomic DNA G+C content was 72.83 mol%. However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305Twas distinguished from closely relatedStreptomycesspecies. Therefore, strain OTB305Trepresents a novel species of the genusStreptomyces, for which the nameStreptomyces otsuchiensissp. nov. is proposed. The type strain is OTB305T(=NBRC 113255T=TBRC 9682T).