Differential expression and autoradiographic localization of atrial natriuretic peptide receptor in spontaneously hypertensive and normotensive rat testes: diminution of testosterone in hypertension.
Differential expression and autoradiographic localization of atrial natriuretic peptide receptor in spontaneously hypertensive and normotensive rat testes: diminution of testosterone in hypertension.
复制标题
自发性高血压和正常血压大鼠睾丸中心房钠尿肽受体的差异表达和放射自显影定位：高血压中睾酮的减少。
DOI：
10.1161/01.hyp.28.5.847
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Pandey,KN
中科院分区：
文献类型：
--
作者：
Kapasi,AA;Kumar,R;Pauly,JR;Pandey,KN
先前的研究表明，利尿激素心钠素（ANP）也调节小鼠和大鼠睾丸间质细胞的类固醇合成反应。在本研究中，我们研究了12周龄的Wistar-Kyoto（WKY）和自发性高血压大鼠（SHR）睾丸隔室中ANP和C型利钠肽（CNP）的特异性受体的分布。我们用125 I-ANP和125 I-CNP作为放射性配体，在冷冻睾丸切片上进行体外放射自显影，定位利钠肽激素家族的受体。高水平的特异性125 I-ANP结合位点主要定位于间质室的间质细胞;其他睾丸细胞未被显著标记。而SHR和WKY睾丸细胞的125 I-CNP结合位点无显著性差异。结合位点的半定量分析表明，WKY睾丸间质细胞的~（125）I-ANP受体结合密度是SHR睾丸间质细胞的9倍。在SHR和WKY的曲细精管中也观察到中等水平的~（125）I-ANP结合，尤其是在精子细胞中，WKY精子细胞的~（125）I-ANP结合比SHR精子细胞高约2.5倍。北方印迹分析表明，鸟苷酸环化酶A型（Npra）特异性mRNA在WKY中的表达水平比SHR睾丸高约2倍。与SHR相比，ANP（1×10− 8 mol/L）刺激正常血压WKY的Leydig细胞产生4 - 5倍的睾酮水平。这些研究结果支持了一个新的生理作用，心钠素在Leydig细胞，在睾丸心钠素受体的表达和睾酮的生产和高血压状态的SHR之间似乎存在一个功能的关系。
Previous studies have shown that the diuretic hormone atrial natriuretic peptide (ANP) also regulates the steroidogenic responsiveness in isolated Leydig cells from mouse and rat testes. In the present study, we examined the distribution of specific receptors for ANP and C-type natriuretic peptide (CNP) in the testicular compartments of 12-week-old Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). We used an in vitro autoradiographic procedure on slide-mounted frozen testicular sections to localize the receptors of the natriuretic peptide hormone family using125I-ANP and125I-CNP as radioligands. A high level of specific125I-ANP binding sites was localized largely in the Leydig cells of the interstitial compartment; other testicular cells were not significantly labeled. On the other hand, no significant difference was observed in125I-CNP binding sites in the testicular cells of SHR and WKY. Semiquantitative analysis of the binding sites indicated that the density of125I-ANP receptor binding in Leydig cells of WKY testis was ninefold higher than in those of SHR testis. A moderate level of125I-ANP binding was also observed in seminiferous tubules, particularly in the spermatids of both SHR and WKY.125I-ANP binding in WKY spermatids was approximately 2.5-fold higher than in SHR spermatids. Northern blot analysis showed that mRNA specific for guanylyl cyclase type A (Npra) was expressed at approximately twofold higher levels in WKY than in SHR testis. ANP (1×10−8mol/L) stimulated fourfold to fivefold increased levels of testosterone production in isolated Leydig cells from normotensive WKY compared with those from SHR. These findings support a new physiological role of ANP in Leydig cells, in which a functional relationship seems to exist between testicular ANP receptor expression and testosterone production and the state of hypertension in SHR.