Development of biodegradable hyper-branched tissue adhesives for the repair of meniscus tears
Development of biodegradable hyper-branched tissue adhesives for the repair of meniscus tears
复制标题
DOI：
10.1016/j.actbio.2015.12.018
复制
发表时间：
2016-03-01
影响因子：
9.7
通讯作者：
Grijpma, D. W.
中科院分区：
文献类型：
--
作者：
Bochynska, A. I.;Van Tienen, T. G.;Grijpma, D. W.
半月板撕裂是膝关节最常见的损伤之一。目前的半月板修复技术具有挑战性，并不能带来完全令人满意的结果。组织粘合剂是一种很有前途的粘合剂，因为它们使用方便，对组织损伤最小。在本研究中，合成了一系列基于聚乙二醇、碳酸三甲酯和柠檬酸的两亲性共聚物，然后用六亚甲基二异氰酸酯对其进行端基处理，形成反应性粘接材料。在搭接剪切粘合试验中测得该网络对牛半月板组织的剪切粘接强度在20~80kPa.网状结构的弹性系数与组成有关，与人体半月板的弹性系数在同一范围内。将牛半月板细胞与不同浓度的明胶孵育7天后，用Alamar Blue染色评价细胞相容性。添加3毫克/毫升的粘合剂对细胞活力无影响，可作为半月板修复的可吸收组织粘合剂。它们具有优异的机械和粘合性能，可以通过改变共聚物的组成进行调整。使用生物可降解组织粘合剂将是一个有趣的选择，但目前可用的粘合剂由于毒性或机械性能较差而不适合使用。在这里，我们描述了新型可生物降解、超支化、粘合共聚物的发展。这些粘合剂在与水接触时固化，形成灵活的网络。其与牛半月板组织的粘附性明显好于临床使用的纤维蛋白胶。固化网状物的拉伸性能与人体半月板的值范围相同。当在培养的细胞中加入生理适量时，没有观察到毒性效应。因此，所提出的材料是用于半月板修复的有趣的可吸收组织粘合剂。(C)2015 Acta Materialia Inc.由爱思唯尔有限公司出版。版权所有。
Meniscus tears are one of the most commonly occurring injuries of the knee joint. Current meniscus repair techniques are challenging and do not bring fully satisfactory results. Tissue adhesives are a promising alternative, since they are easy to apply and cause minimal tissue trauma.In this study, a series of amphiphilic copolymers based on polyethylene glycol, trimethylene carbonate and citric acid were synthesized and subsequently end-functionalized with hexamethylene diisocyanate to form reactive adhesive materials.The shear adhesive strength of the networks to bovine meniscus tissue measured in a lap-shear adhesion test ranged between 20 and 80 kPa, which was better than for fibrin glue (10 kPa). The elastic modulus of the networks depended on composition and was in the same range as that of human meniscus. Cell compatibility was assessed using Alamar Blue staining after incubation of the bovine meniscus cells with different concentrations of the glues for 7 days. Cell viability was not affected after adding up to 3 mg of the adhesive/mL of medium.The proposed materials are suitable candidates to be used as resorbable tissue adhesives for meniscus repair. They have excellent mechanical and adhesive properties that can be adjusted by varying the composition of the copolymers.Statement of SignificanceMeniscal tears often occur and current treatment strategies do not bring fully satisfactory results. Use of biodegradable tissue adhesives would be an interesting option, but currently available adhesives are not suited due to toxicity or poor mechanical properties.Here, we describe the development of novel biodegradable, hyper-branched, adhesive copolymers. These adhesives cure upon contact with water forming flexible networks. Their adhesion to bovine meniscus tissue was significantly better than that of clinically used fibrin glue. The tensile properties of the cured networks were in the same range of values of the human meniscus. When physiologically relevant amounts were added to cells in culture, not toxic effects were observed. Therefore, the proposed materials are interesting resorbable tissue adhesives for meniscus repair. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.