Pocket Epithelium in the Pathological Setting for HMGB1 Release
Pocket Epithelium in the Pathological Setting for HMGB1 Release
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DOI：
10.1177/0022034510385688
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发表时间：
2011-02-01
影响因子：
7.6
通讯作者：
Izumi, Y.
中科院分区：
文献类型：
--
作者：
Ebe, N.;Hara-Yokoyama, M.;Izumi, Y.
高迁移率组盒-1 （HMGB1）蛋白在细胞核中作为转录因子，当释放到细胞外液中时也作为促炎细胞因子。据报道，牙周病患者的龈沟液中存在较高水平的HMGB1。由于牙周袋内细菌的增殖与牙周疾病的恶化密切相关，因此假设牙周袋导致HMGB1的释放。炎症龈的免疫组化染色显示HMGB1在袋上皮中只从细胞核向胞质脱位，而在龈上皮中主要存在于细胞核中。丁酸是牙周病细菌在牙周袋内的细胞外代谢物，通过诱导人牙龈上皮细胞系坏死，诱导HMGB1的被动释放。因此，牙周上皮可能为细菌侵袭释放HMGB1提供了独特的病理环境。简称：HMGB1，高迁移率组盒1；肿瘤坏死因子；CHX,环己酰亚胺;PI：碘化丙啶；ROS，活性氧；组蛋白去乙酰化酶。
High-mobility group box-1 (HMGB1) protein acts as a transcription factor in the nucleus and also as a pro-inflammatory cytokine when released into extracellular fluids. The presence of higher levels of HMGB1 is reported in the gingival crevicular fluid from periodontal patients. Since the proliferation of bacteria within the periodontal pocket is closely involved in the exacerbation of periodontal disease, it is hypothesized that the periodontal pocket causes the release of HMGB1. Immunohistochemical staining of inflamed gingiva revealed that HMGB1 is exclusively dislocated from the nucleus to the cytoplasm in the pocket epithelium, whereas it is mainly present in the nucleus in the gingival epithelium. Butyric acid, an extracellular metabolite from periodontopathic bacteria populating the periodontal pocket, induced the passive release of HMGB1 as a result of eliciting necrosis in the human gingival epithelial cell line. Thus, the periodontal epithelium may provide a unique pathological setting for HMGB1 release by bacterial insult. Abbreviations: HMGB1, high-mobility group box-1; TNF-alpha, tumor necrosis factor-alpha; CHX, cycloheximide; PI, propidium iodide; ROS, reactive oxygen species; HDAC, histone deacetylase.