THE MOLECULAR-GENETICS OF THE BITHORAX COMPLEX OF DROSOPHILA - CIS-REGULATION IN THE ABDOMINAL-B DOMAIN
THE MOLECULAR-GENETICS OF THE BITHORAX COMPLEX OF DROSOPHILA - CIS-REGULATION IN THE ABDOMINAL-B DOMAIN
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DOI：
10.1002/j.1460-2075.1990.tb07876.x
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发表时间：
1990-12-01
期刊：
影响因子：
11.4
通讯作者：
LEWIS, EB
中科院分区：
文献类型：
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作者：
CELNIKER, SE;SHARMA, S;LEWIS, EB
在果蝇中，双胸复合体（BX-C）的Abdominal-B（Abd-B）结构域跨越超过100 kb，并且负责指定成人腹部节5（A5）至9（A9）（包括A5和A9）的身份，以及相应地，胚胎中枢神经系统的neuormeres 10-14的身份。 该结构域由编码两种蛋白质ABD-BI（54 kd）和ABD-BII（36 kd）的区域以及从腹下-5（iab-5）延伸至iab-9（包括端值）的顺式调节区域组成。 我们已经使用单克隆抗ABD-B抗体来推断iab-8中的突变体消除了神经节10-13中ABD-BI的表达，并且iab-9中的突变体消除了神经节14中ABD-BII的表达。还在纯合子中分析ABD-B表达的（i）涉及iab-5、iab-6和iab-7区域的功能丧失突变体，（ii）功能获得突变体Miscadastral色素沉着（Mcp）和Superabdominal（Sab），和（iii）反式调节子Polycomb（Pc）。 ABD-B表达沿着前后轴与顺式调节区的染色体顺序共线。 增强相关的iab-6和iab-7突变体的行为表明，增强子样区域，iab-5，也可能是iab-6，可以共享之间的Abd-A和Abd结构域。 这种共享被认为是在进化过程中保持基因复合体完整的一个因素。
In Drosophila the Abdominal-B (Abd-B) domain of the bithorax complex (BX-C) spans over 100 kb and is responsible for specifying the identities of adult abdominal segments five (A5) to nine (A9), inclusive, and correspondingly, neuormeres 10-14 of the embryonic central nervous system. The domain consists of a region coding for two proteins, ABD-BI (54 kd) and ABD-BII (36 kd) and cis-regulatory regions extending from infra-abdominal-5 (iab-5) to iab-9, inclusive. We have used a monoclonal anti-ABD-B antibody to infer that mutants in iab-8 eliminate the expression of ABD-BI in neuromeres 10-13, inclusive, and that mutants in iab-9 eliminate expression of ABD-BII in neuromere 14. ABD-B expression is also analyzed in homozygotes for (i) loss-of-function mutants involving the iab-5, iab-6 and iab-7 regions, (ii) gain-of-functions mutants Miscadastral pigmentation (Mcp) and Superabdominal (Sab), and (iii) a trans-regulator, Polycomb (Pc). ABD-B expression along the antero-posterior axis is colinear with the chromosomal order of the cis-regulatory regions. The behavior of rearrangement-associated iab-6 and iab-7 mutants suggest that the enhancer-like region, iab-5, and possibly also iab-6, may be shared between the Abd-A and Abd domains. Such sharing is proposed as a factor that tends to keep gene complexes intact during evolution.