Calorie restriction improves whole-body glucose disposal and insulin resistance in association with the increased adipocyte-specific GLUT4 expression in Otsuka Long-Evans Tokushima Fatty rats
Calorie restriction improves whole-body glucose disposal and insulin resistance in association with the increased adipocyte-specific GLUT4 expression in Otsuka Long-Evans Tokushima Fatty rats
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DOI：
10.1016/j.abb.2005.01.010
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发表时间：
2005-04-15
影响因子：
3.9
通讯作者：
Lee, W
中科院分区：
文献类型：
--
作者：
Park, SY;Choi, GH;Lee, W
热量限制（CR）已被证明可以改善外周胰岛素抵抗和2型糖尿病的动物模型。然而，CR对胰岛素敏感组织中GLUT 4表达和易位的确切机制尚未得到很好的阐明。在本研究中，我们研究了CR对葡萄糖转运蛋白4（GLUT 4）的表达，GLUT 4易位，和葡萄糖转运活性的大冢长埃文斯德岛脂肪（OLETF）大鼠和对照（LETO）大鼠脂肪组织中的影响。CR（70%饱足组）可改善OLETF大鼠的高血糖和糖耐量减低（IGT）。在骨骼肌中，GLUT 4和GLUT 1的表达水平在LETO和OLETF大鼠之间没有显著差异，并且不受CR的影响。相反，OLETF大鼠脂肪组织中GLUT 4的表达水平显著降低，但CR显著增加。胰岛素刺激的GLUT 4募集也通过CR在OLETF大鼠脂肪细胞中得到改善。胰岛素刺激的2-脱氧葡萄糖（2DG）摄取显着增加脂肪细胞从CR OLETF大鼠，与饱食OLETF大鼠相比。总而言之，这些结果表明CR改善了OLETF大鼠的全身葡萄糖处理和胰岛素抵抗，并且这些作用可能与脂肪细胞特异性GLUT 4表达的增加有关。(c)2005年由Elsevier Inc.出版
Calorie restriction (CR) has been shown to improve peripheral insulin resistance and type 2 diabetes in animal models. However, the exact mechanism of CR on GLUT4 expression and translocation in insulin-sensitive tissues has not been well elucidated. In the present study, we examine the effect of CR on the expression of glucose transporter 4 (GLUT4), GLUT4 translocation, and glucose transport activity in adipose tissue from Otsuka Long-Evans Tokushima Fatty (OLETF) rat and control (LETO) rats. CR (70% of satiated group) ameliorated hyperglycemia and improved impaired glucose tolerance (IGT) in OLETF rats. In skeletal muscle, the expression levels of GLUT4 and GLUT1 were not significantly different between LETO and OLETF rats, and were not affected by CR. By contrast, the expression level of GLUT4 was markedly decreased in the adipose tissue of OLETF rats, but was dramatically increased by CR. The GLUT4 recruitment stimulated by insulin was also improved in OLETF rat adipocytes by CR. The insulin-stimulated 2-deoxyglucose (2DG) uptake was significantly increased in adipocytes from the CR OLETF rats, as compared with the satiated OLETF rats. Taken together, these results suggest that CR improves whole body glucose disposal and insulin resistance in OLETF rats, and that these effects may associate with the increased adipocyte-specific GLUT4 expression. (c) 2005 Published by Elsevier Inc.