Serum-induced collagen gel contraction.
Serum-induced collagen gel contraction.
复制标题
血清诱导的胶原凝胶收缩。
DOI：
10.1007/bf00180947
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Chakrabarti,B
中科院分区：
文献类型：
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作者：
Akiba,J;Kakehashi,A;Ueno,N;Tano,Y;Chakrabarti,B
·目的：为了了解疾病相关的玻璃体凝胶收缩的分子事件，研究了血清组分对胶原蛋白的影响。·方法：将牛玻璃体或真皮胶原与转氨酶（TG;因子XIIIa）和纤连蛋白（FN）的混合物一起孵育，并通过凝胶电泳监测胶原的生化变化。此外，监测血清诱导的胶原凝胶体积变化。·结果：凝胶电泳显示一个新的高分子量带（Mr 240 000），推测是由于胶原蛋白肽和FN的分子间交联。血清组分也显示出引起胶原蛋白凝胶体积的显著减少。·结论。胶原凝胶收缩可归因于TG催化的胶原-FN-胶原交联。
• Purpose: To understand the molecular events underlying disease-related vitreous gel contraction, the effect of serum components on collagen was investigated. • Methods: Bovine vitreous or dermal collagen was incubated with a mixture of transglutaminase (TG; factor XIIIa) and fibronectin (FN), and the biochemical changes of collagen were monitored by gel electrophoresis. In addition, serum-induced changes in the volume of the collagen gel were monitored. • Results: Gel electrophoresis revealed a new high-molecular-weight band (Mr240 000) presumably due to intermolecular cross-links of collagen peptides and FN. The serum components also were shown to cause a significant decrease in the volume of the collagen gel. • Conclusion. Collagen gel contraction could be attributed to the collagen-FN-collagen cross-links catalyzed by TG.