ESTRADIOL-17-BETA PRODUCTION IN AMAGO SALMON (ONCORHYNCHUS-RHODURUS) OVARIAN FOLLICLES - ROLE OF THE THECAL AND GRANULOSA-CELLS
ESTRADIOL-17-BETA PRODUCTION IN AMAGO SALMON (ONCORHYNCHUS-RHODURUS) OVARIAN FOLLICLES - ROLE OF THE THECAL AND GRANULOSA-CELLS
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DOI：
10.1016/0016-6480(82)90122-8
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
NAGAHAMA, Y
中科院分区：
文献类型：
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作者：
KAGAWA, H;YOUNG, G;NAGAHAMA, Y
分离鲑鱼卵母细胞的滤泡膜细胞和颗粒细胞层有助于评估它们在雌二醇-17β中的作用。通过体外孵化的方法生产。将完整卵泡(卵泡层完整的卵母细胞)、污染10%颗粒卵泡层的卵膜层、纯颗粒卵泡层和放射状带卵泡层以及卵膜层-颗粒层共培养的4种卵泡标本，在有或没有部分纯化的三文鱼促性腺激素(SG-G100)存在或不存在的情况下进行培养。雌二醇-17β。用放射免疫法测定培养液中的睾酮水平。SG-G100(0.1、1微克/毫升)刺激雌二醇-17β。由完整的卵泡和共培养的制剂产生，但不是由分离的膜或颗粒层产生；这表明这两层都是促性腺激素刺激的雌二醇-17β所必需的。制作。SG-G100极大地刺激了卵泡层的睾酮生成(最高可达基础对照的80倍)，但对其他卵泡制剂的睾酮生成的刺激作用很小。颗粒层与外源性睾酮(0.01、1微克/毫升)孵育，或与睾酮和SG-G100(1微克/毫升)共同孵育，可导致睾酮水平升高。两种处理之间没有显著差异，提示促性腺激素不能刺激睾酮转化为雌二醇-17β。离体膜层用睾酮孵育产生的雌二醇-17β相对较少，比同等条件下的颗粒层少7-8倍。提出了卵泡雌激素产生的两细胞型模型，膜层可能参与了雌二醇-17β的产生。通过合成雄激素产生的，雄激素被转移到颗粒层并芳香化为雌二醇-17β。
Separation of the follicular thecal cell and granulosa cell layers of oocytes of the amago salmon (O. rhodurus) facilitated assessment of their roles in estradiol-17.beta. production by an in vitro incubation method. Four different follicular preparations, intact follicles (oocytes with complete follicle layers), thecal layers contaminated with < 10% granulosa layers, pure granulosa layers and zona radiata, and thecal layer-granulosa layer co-cultures, were incubated in the presence or absence of partially purified chinook salmon gonadotropin (SG-G100). Estradiol-17.beta. and testosterone levels in the medium were measured by specific radioimmunoassay. SG-G100 (0.1, 1 .mu.g/ml) stimulated estradiol-17.beta. production by intact follicles and co-cultured preparations, but not by the isolated thecal or granulosa layers; this indicates that both layers are necessary for gonadotropin-stimulated estradiol-17.beta. production. SG-G100 greatly stimulated testosterone production by thecal layers (up to 80 times basal control values) but only slightly stimulated testosterone production by the other follicular preparations. Incubation of granulosa layers with exogenous testosterone (0.01, 1 .mu.g/ml), or with testosterone and SG-G100 (1 .mu.g/ml) together, resulted in elevated testosterone levels. No significant difference was found between the 2 treatments, suggesting that gonadotropin did not stimulate the conversion of testosterone to estradiol-17.beta.. Isolated thecal layers incubated with testosterone produced relatively small amounts of estradiol-17.beta., 7-8 times less than granulosa layers under the same conditions. A 2-cell type model for the production of follicular estrogens is suggested, the thecal layer possibly contributing to estradiol-17.beta. production by synthesizing androgens which are transferred to the granulosa layer and aromatized to estradiol-17.beta.