Effects of chlorimuron-ethyl, imazethapyr, and propachlor on free amino acids and protein formation in Vicia faba L
Effects of chlorimuron-ethyl, imazethapyr, and propachlor on free amino acids and protein formation in Vicia faba L
复制标题
DOI：
10.1021/jf960771f
复制
发表时间：
1997-09
影响因子：
6.1
通讯作者：
L. Scarponi;M. E. Younis;A. Standardi;N. M. Hassan;L. Martinetti
中科院分区：
文献类型：
--
作者：
L. Scarponi;M. E. Younis;A. Standardi;N. M. Hassan;L. Martinetti
经氯磺隆、咪草胺或丙草胺处理后，蚕豆的蛋白质含量下降，游离氨基酸含量发生变化。绿磺隆和咪草胺降低了土壤中的缬氨酸、亮氨酸、异亮氨酸和全氮含量，而增加了可溶性氮的含量。伴随这些变化的是乙酰羟基酸合成酶(AHAS；EC 4.1.3.18)、亚硝酸盐还原酶(NIR；EC 1.7.7.1)、谷氨酰胺合成酶(GS；EC 6.3.1.2)和谷氨酸合成酶(NADH-GOGAT；EC 1.4.1.14)活性降低。在试验期间，氯磺隆和咪草胺对GS活性的抑制作用先于NIR和AHAs，而丙草胺对GS活性的抑制作用则相反。因此，蛋白质形成减少和游离氨基酸谱的紊乱可能是由于氯磺隆或咪唑烟处理后抑制AHAs和处理后抑制NIR所致。
Decreases in protein and changes in the free amino acid profile were ascertained in Vicia faba L. treated with chlorimuron-ethyl, imazethapyr, or propachlor. Valine, leucine, isoleucine, and total N were decreased by chlorimuron-ethyl and imazethapyr, while total soluble N was increased by all treatments. These changes were accompanied by decreases of the activities of acetohydroxy acid synthase (AHAS; EC 4.1.3.18), nitrite reductase (NiR; EC 1.7.7.1), glutamine synthetase (GS; EC 6.3.1.2), and glutamate synthase (NADH-GOGAT; EC 1.4.1.14). During the experimental period, the inhibition of GS activity preceded that of NiR and followed that of AHAS in samples treated with chlorimuron-ethyl or imazethapyr, while a reverse sequence was apparent in samples treated with propachlor. Therefore, the reduction in protein formation and the disturbance in free amino acid profile appears to be due to inhibition of AHAS following treatment with chlorimuron-ethyl or imazethapyr and to inhibition of NiR following treatme...