The neuronal composition of area 17 of rat visual cortex. III. Numerical considerations
The neuronal composition of area 17 of rat visual cortex. III. Numerical considerations
复制标题
大鼠视觉皮层17区的神经元组成。
DOI：
10.1002/cne.902380303
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发表时间：
1985
影响因子：
2.5
通讯作者：
K. Harriman
中科院分区：
文献类型：
--
作者：
A. Peters;Daniel A. Kara;K. Harriman
用灌注固定的脑组织观察了大鼠视皮层17区的神经元群。将组织渗透并包埋在塑料中，以便可以通过光学显微镜和电子显微镜检查相同的神经元。在这些制备物中，区域17为1.49 mm厚，并且通过体视学程序计算出在1 mm 2的皮质表面下存在约120，000个神经元。如果假设大鼠每个半球的17区占据7.1至9.4平方毫米的皮质表面，那么在每个半球中，该区域包含850，000至1，128，000个神经元。这些神经元中85%是锥体细胞，15%是非锥体细胞。大约三分之一的非锥体细胞出现在I层和VIb层，这两层都只含有这种神经元。其余三分之二的非锥体细胞位于II-VIa层。在这些细胞层中，可以将双极细胞与其他类型的非锥体细胞区分开来，并且在这两种非锥体细胞群中的每一种中，都可以识别小神经元和大神经元。非锥体细胞的最大浓度出现在第II/III层。为了证实体视学数据的有效性，对V层中的中型和大型锥体细胞进行了直接计数。
The neuronal population of area 17 of rat visual cortex has been examined by using tissue from brains fixed by perfusion. The tissue was osmicated and embedded in plastic so that the same neurons could be examined by both light and electron microscopy. In these preparations area 17 was 1.49 mm thick and by stereological procedures it was calculated that there are about 120,000 neurons beneath 1 mm2 of cortical surface. If one assumes area 17 in each hemisphere of the rat to occupy between 7.1 and 9.4 mm2 of cortical surface, then in each hemisphere the area contains between 850,000 and 1,128,000 neurons. Of these neurons 85% are pyramidal cells and 15% are nonpyramidal cells. About one‐third of the nonpyramidal cells occur in layers I and VIb, both of which contain only this kind of neuron. The remaining two‐thirds of the nonpyramidal cells are in layers II‐VIa. Within these layers it has been possible to differentiate bipolar cells from other types of nonpyramidal cells and in each of these two nonpyramidal cell groups to recognize both small and large neurons. The greatest concentration of nonpyramidal cells occurs in layer II/III. To confirm the validity of the stereologically derived data direct counts were made of the medium and large pyramidal cells in layer V.