Evaluating auxin distribution in tomato (Solanum lycopersicum) through an analysis of the PIN and AUX/LAX gene families
Evaluating auxin distribution in tomato (Solanum lycopersicum) through an analysis of the PIN and AUX/LAX gene families
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DOI：
10.1111/j.1365-313x.2011.04895.x
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发表时间：
2012-05-01
期刊：
影响因子：
7.2
通讯作者：
Catala, Carmen
中科院分区：
文献类型：
--
作者：
Pattison, Richard J.;Catala, Carmen
生长素分布的时空调控在植物生长发育调控中起着关键作用，关于影响营养组织中生长素库和生长素梯度的机制已有许多研究，尤其是在拟南芥中。例如，由PIN和AUX/LAX蛋白介导的生长素极性运输是控制生长素分布的中心。相反，尽管生长素调节果实发育的许多方面，包括果实形成、膨大、成熟和脱落，但对生长素分布的动态及其在果实组织内部和之间运输的分子基础知之甚少。此外，关于拟南芥以外物种在营养和生殖发育过程中生长素通量的关键调节因子的功能信息也很少。为了解决这些问题，我们研究了番茄果实发育过程中生长素的时空分布以及PIN和AUX/LAX基因家族的功能。在果实生长早期，生长素的浓度差异明显，内部组织中的生长素水平高于果皮中的生长素水平，生长素的积累模式取决于极地运输。已鉴定出10个番茄PIN基因(SLPIN1~10)和5个AUX/LAX基因(SLLAX1~5)，表现出组织和发育阶段特异性的异质性表达模式。RNAi介导的SLPIN4和SLPIN3共沉默不影响果实发育，这表明PIN蛋白功能冗余，但确实导致了营养表型，并揭示了这些基因在番茄新梢构型调控中的作用。
The temporal and spatial control of auxin distribution has a key role in the regulation of plant growth and development, and much has been learnt about the mechanisms that influence auxin pools and gradients in vegetative tissues, particularly in Arabidopsis. For example polar auxin transport, mediated by PIN and AUX/LAX proteins, is central to the control of auxin distribution. In contrast, very little information is known about the dynamics of auxin distribution and the molecular basis of its transport within and between fruit tissues, despite the fact that auxin regulates many aspects of fruit development, which include fruit formation, expansion, ripening and abscission. In addition, functional information regarding the key regulators of auxin fluxes during both vegetative and reproductive development in species other than Arabidopsis is scarce. To address these issues, we have investigated the spatiotemporal distribution of auxin during tomato (Solanum lycopersicum) fruit development and the function of the PIN and AUX/LAX gene families. Differential concentrations of auxin become apparent during early fruit growth, with auxin levels being higher in internal tissues than in the fruit pericarp and the pattern of auxin accumulation depended on polar transport. Ten tomato PIN (SlPIN1 to 10) and five AUX/LAX (SlLAX1 to 5) genes were identified and found to display heterogeneous expression patterns, with tissue and developmental-stage specificity. RNAi-mediated co-silencing of SlPIN4 and SlPIN3 did not affect fruit development, which suggested functional redundancy of PIN proteins, but did lead to a vegetative phenotype, and revealed a role for these genes in the regulation of tomato shoot architecture.