Denitrification by yeasts and occurrence of cytochrome P450nor in Trichosporon cutaneum.
Denitrification by yeasts and occurrence of cytochrome P450nor in Trichosporon cutaneum.
复制标题
皮肤毛孢子菌中酵母的反硝化和细胞色素 P450nor 的出现。
DOI：
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发表时间：
1998
影响因子：
2.1
通讯作者：
Takashi Nakase
中科院分区：
文献类型：
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作者：
Sayuri Tsuruta;Naoki Takaya;Li Zhang;Hirofumi Shoun;Kenji Kimura;M. Hamamoto;Takashi Nakase
对不同属的酵母菌进行了反硝化活性的筛选，并筛选出了皮肤毛孢霉、福州费氏酵母和念珠菌等几株酵母。被发现具有将亚硝酸盐转化为一氧化二氮的独特活性。在这些酵母菌的无细胞提取物中检测到异化亚硝酸盐还原酶(NIR)或一氧化氮还原酶(NOR)活性。分光光度分析和Western印迹分析表明，皮肤红豆杉含有NOR细胞色素P450类型。这是第一个表明反硝化作用也分布在酵母中的报告，尽管它们的系统不完整，只能将亚硝酸盐还原为一氧化二氮。
Yeasts of various genera were screened for denitrifying activity, and several yeast strains such as Trichosporon cutaneum, Fellomyces fuzhouensis, and Candida sp. were found to exhibit distinct activities to convert nitrite to nitrous oxide. Dissimilatory nitrite reductase (Nir) or nitric oxide reductase (Nor) activities were detected in the cell-free extracts of these yeasts. Spectrophotometric as well as Western blot analyses showed that T. cutaneum contains Nor of the cytochrome P450 type. This is the first report that shows that denitrification is also distributed among yeasts although their systems are incomplete, only capable of reducing nitrite to nitrous oxide.