CLINICAL ANALYSIS BY H-1 SPIN-ECHO NMR .2. OXIDATION OF INTRACELLULAR GLUTATHIONE AS A CONSEQUENCE OF PENICILLAMINE THERAPY IN RHEUMATOID-ARTHRITIS
CLINICAL ANALYSIS BY H-1 SPIN-ECHO NMR .2. OXIDATION OF INTRACELLULAR GLUTATHIONE AS A CONSEQUENCE OF PENICILLAMINE THERAPY IN RHEUMATOID-ARTHRITIS
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DOI：
10.1021/jm00089a026
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发表时间：
1992-05-29
影响因子：
7.3
通讯作者：
STURROCK, RD
中科院分区：
文献类型：
--
作者：
REGLINSKI, J;SMITH, WE;STURROCK, RD
自旋回波核磁共振分析用于监测青霉胺对类风湿性关节炎患者在12周治疗期间获得的完整红细胞的影响。该结果与先前报道的化合物的体外效应进行了比较(McKay, c.n.n.等人，biochem。Biophys。学报，1986,888,30-35)。第12周临床评估时，将20例患者分为有反应组和无反应组。应答组细胞内谷胱甘肽氧化程度高于无应答组（P < 0.01）。在治疗开始后对两组患者进行初步评估时的回顾性分析表明，无反应组细胞内谷胱甘肽的减少明显多于反应组（P < 0.02）。据推测，青霉胺刺激细胞防御细胞膜氧化以牺牲胞质谷胱甘肽为代价。这项初步研究表明，红细胞谷胱甘肽的自旋回声核磁共振分析可以作为青霉胺治疗临床反应的早期指标。
Spin echo NMR analysis is used to monitor the effect of penicillamine on intact erythrocytes obtained from patients suffering from rheumatoid arthritis during at 12-week period of therapy. The results are compared to the previously reported in vitro effects of the compound (McKay, C. N. N.; et al, Biochim. Biophys. Acta 1986, 888, 30-35). At clinical assessment at week 12, the 20 patients were divided into responder and nonresponder groups. The intracellular glutathione in the responder group is more oxidized (P < 0.01) than in the nonresponder group. A retrospective analysis of the two patient groups at the initial assessment following the commencement of therapy indicated that in the nonresponder group intracellular glutathione was significantly more reduced (P < 0.02) than in the responder group. It is postulated that penicillamine stimulates cellular defense against the oxidation of the cell membrane at the expense of cytosolic glutathione. This initial study suggests that spin-echo NMR analysis of erythrocyte glutathione can act as an early indicator of a clinical response to penicillamine therapy.