Coenzyme Q10 Decreases TNF-α and IL-2 Secretion by Human Peripheral Blood Mononuclear Cells
Coenzyme Q10 Decreases TNF-α and IL-2 Secretion by Human Peripheral Blood Mononuclear Cells
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DOI：
10.3177/jnsv.56.77
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发表时间：
2010-02-01
影响因子：
1.6
通讯作者：
Salman, Hertzel
中科院分区：
文献类型：
--
作者：
Bessler, Hanna;Bergman, Michael;Salman, Hertzel
辅酶Q10（CoQ 10）对人体健康的有益作用通过各种机制发生，包括免疫调节的可能性。因此，本研究的目的是检测辅酶Q10对人外周血单个核细胞（PBMC）细胞因子产生和超氧阴离子产生的体外作用。2 × 10（6）/mL，从1.9名志愿者中获得的PBMC在不加或加0.6、1.25、2.5和5.0 μ m辅酶Q10的情况下孵育24小时。检测了以下细胞因子的产生：IL-1 β、IL-1 ra、IL-6、IL-10、IL-2和IFN γ。通过将4 × 10（6）/mL细胞与辅酶Q10和2 × 10（-3）mM佛波醇甲磺酸酯乙酸酯（PMA）孵育60分钟来检测超氧阴离子的产生。PBMC的促炎细胞因子IL-1 β、IL-6和IFN γ以及抗炎细胞因子IL-1 ra和IL-10的产生不受辅酶Q10的影响，而当细胞与0.6和1.25 μ M辅酶Q10一起孵育时，TNF α分泌显著降低。另一方面，增加剂量的辅酶Q10引起轻微的，但统计学上显著的IL-2分泌抑制。PBMC产生的超氧阴离子在与或不与浓度为0.3和5.0 μ M的辅酶Q10孵育的细胞之间没有显著差异。结果提示，辅酶Q10对PBMC产生细胞因子有一定的影响，这与其调节人体免疫功能的能力有关。
The beneficial effect of coenzyme Q10 (CoQ10) on human health occurs through various mechanisms including the possibility of immunomodulation. Therefore, the purpose of Study was to examine the in vitro effect of CoQ10 on cytokine production and superoxide anion generation by human peripheral blood mononuclear cells (PBMC). 2 X 10(6)/mL, PBMC obtained from 1.9 volunteers were incubated for 24 h without or with 0.6, 1.25, 2.5 and 5.0 mu m of CoQ10. The production of the following cytokines were examined: IL-1 beta, IL-1ra, IL-6, IL-10, IL-2 and IFN gamma. Superoxide anion production was examined by incubation of 4 X 10(6)/mL cells with CoQ10 and 2 X 10(-3) mM phorbol merystate acetate (PMA) for 60 min. The production of the proinflammatory cytokines IL-1 beta, IL-6 and IFN gamma and that of the anti-inflammatory cytokines IL-1ra and IL-10 by PBMC was not affected by CoQ10, whereas TNF alpha secretion was significantly decreased when the cells were incubated with 0.6 and 1.25 mu M of CoQ10. On the other hand, increasing doses of CoQ10 caused mild, but statistically significant inhibition of IL-2 secretion. The generation of superoxide anion by PBMC did not differ significantly between cells incubated with or without CoQ10 at concentrations between 0.3 and 5.0 mu M. The results suggest that CoQ10 exerts a certain effect on cytokine production by PBMC related to its capacity to modulate human immune function.