OF PROTONS OR PROTEINS - A BEAMS A BEAM FOR A THAT (BURNS,O.S.)
OF PROTONS OR PROTEINS - A BEAMS A BEAM FOR A THAT (BURNS,O.S.)
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DOI：
10.1007/bf01384871
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发表时间：
1988-01-01
期刊：
影响因子：
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通讯作者：
FENN, JB
中科院分区：
文献类型：
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作者：
MENG, CK;MANN, M;FENN, JB
质量分析已经进行了由电喷雾（ES）源从稀溶液的蛋白质分子的分子量（M）的范围从5000到近40000的离子。每个光谱包括对应于多电荷完整母体物质的一系列峰。在这些实验中，每个峰的离子与其相邻的相邻峰的离子相差一个单位电荷H+。每个离子的最大电荷数一般随母体分子的分子量而增加，在醇脱氢酶的情况下达到45的值，atM=39830是本研究中最大的物种。因此，m/z的结果值在简单的四极质量过滤器的范围内，其标称质量上限为1500道尔顿！ES源的直接应用是对具有生物化学重要性的大的易碎分子进行质谱分析。但它产生的多电荷离子是实验室场景的新人，构成了有趣的研究课题。
Mass analyses have been carried out on ions produced by an Electrospray (ES) source from dilute solutions of protein molecules with molecular weights (M) in the range from 5000 to nearly 40000. Each spectrum comprises a sequence of peaks corresponding to multiply charged intact parent species. The ions of each peak differ from those of their adjacent neighbors by one unit charge, H+in these experiments. The maximum number of charges per ion generally increases with the molecular weight of the parent molecule, reaching a value of 45 in the case of alcohol dehydrogenase, atM=39830 the largest species in this study. Thus the resulting values ofm/zare within reach of a simple quadrupole mass filter whose nominal upper mass limit is 1500 daltons! The immediate application for the ES source is in mass spectrometric analysis of large fragile molecules of biochemical importance. But the multiply charged ions it produces are newcomers to the laboratory scene that constitute interesting subjects for study.