Very high prevalence of TT virus (TTV) infection in general population of Japan revealed by a new set of PCR primers
Very high prevalence of TT virus (TTV) infection in general population of Japan revealed by a new set of PCR primers
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DOI：
10.1016/s1386-6346(98)00068-0
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发表时间：
1998-10-01
影响因子：
4.2
通讯作者：
Mishiro, S
中科院分区：
文献类型：
--
作者：
Takahashi, K;Hoshino, H;Mishiro, S
TT病毒（TTV）是一种肝炎病毒的明确证据仍然未见，尽管它起源于肝炎患者。TTV的流行病学研究或多或少受到诊断系统不成熟的限制。我们寻求能比以前报道的更有效地检测TTV DNA的PCR系统，并发现用命名为T801和T935的新引物进行的PCR比用已公布的引物NG 061和NG 063进行的PCR灵敏度高10-100倍。令人惊讶的是，T801/T935的新PCR系统在100名到我们医院进行健康筛查的人中鉴定出TTV DNA的92%，而NG 061/NG 063的鉴定率为23%。TTV DNA阴性和阳性个体的肝功能检查值相当，但前者的平均年龄比后者年轻：29.7 +/- 6.9岁对41.4 +/- 14.7岁（P < 0.05）。我们的研究结果表明，TTV的无症状携带者在日本的成年人中很常见。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
Clear evidence for TT virus (TTV) to be a hepatitis virus remains unseen despite its origination from a hepatitis patient. Epidemiological studies on TTV have been limited more or less by the prematurity of the diagnostic systems. We sought after PCR systems that could detect TTV DNA more efficiently than those reported previously, and found that a PCR with new primers designated T801 and T935 was 10-100 times more sensitive than that with published primers NG061 and NG063. To our surprise, TTV DNA was identified by the new PCR system with T801/T935 in 92% of 100 individuals who visited our hospital for health screening, compared to 23% with NG061/NG063. Liver function test values were comparable between TTV DNA-negative and positive individuals, but mean age was younger in the former than in the latter: 29.7 +/- 6.9 versus 41.4 +/- 14.7 years (P < 0.05). Our results suggest that asymptomatic carriers of TTV are very common in general population of adults in Japan. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.