Adoptive transfer of T regulatory cells inhibits lipopolysaccharide-induced inflammation in fetal brain tissue in a late-pregnancy preterm birth mouse model
Adoptive transfer of T regulatory cells inhibits lipopolysaccharide-induced inflammation in fetal brain tissue in a late-pregnancy preterm birth mouse model
复制标题
T调节细胞的过继转移抑制妊娠晚期早产小鼠模型中脂多糖诱导的胎儿脑组织炎症
DOI：
10.1002/cbin.10710
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发表时间：
2017-02-01
影响因子：
3.9
通讯作者：
Liu，Li
中科院分区：
文献类型：
--
作者：
Wang，Fan;Xiao，Mi;Liu，Li
为探讨调节性T细胞（Treg）对脂多糖（LPS）刺激的胎鼠脑组织炎症反应的影响，将孕鼠Treg转移到模型鼠体内，检测胎鼠脑组织中Foxp 3、IL-6、CD 68的表达水平。（小胶质细胞的标志物）和Toll样受体4（TLR-4）在胎儿脑组织中进行了评估。采用聚合酶链反应和免疫印迹法检测Foxp 3、IL-6和TLR-4的表达;采用免疫化学法检测CD 68的表达水平。母体LPS给药显著诱导胎脑中Foxp 3、IL-6、TLR-4和CD 68的表达，过继转移TGFAP显著降低了表达水平的增加。母体LPS暴露显著诱导围产期脑组织中的炎症，并且TdR负调节这种LPS诱导的炎症。
To evaluate the effect of regulatory T cells (Tregs) on the inflammation resulting from lipopolysaccharide (LPS) challenge in prenatal brain tissue, Tregs isolated from pregnant mice were transferred into model mice, and the expression levels of fork head family transcription factor (Foxp3), interleukin‐6 (IL‐6), CD68 (a marker of microglia), and toll‐like receptor 4 (TLR‐4) were assessed in the fetal brain tissue. Foxp3, IL‐6, and TLR‐4 expression were detected by polymerase chain reaction and Western blot; CD68 expression level was detected using immunochemical analysis. Foxp3, IL‐6, TLR‐4, and CD68 expressions in fetal brain were significantly induced by maternal LPS administration, and the increased expression levels were markedly reduced by adoptive transfer of Tregs. Maternal LPS exposure significantly induced inflammation in perinatal brain tissue, and Tregs negatively regulated this LPS‐induced inflammation.