Edited transcripts compete with unedited mRNAs for trans-acting editing factors in higher plant chloroplasts
Edited transcripts compete with unedited mRNAs for trans-acting editing factors in higher plant chloroplasts
复制标题
DOI：
10.1016/s0378-1119(01)00545-5
复制
发表时间：
2001-07-11
期刊：
影响因子：
3.5
通讯作者：
Hanson, MR
中科院分区：
文献类型：
--
作者：
Reed, ML;Lyi, SM;Hanson, MR
Chloroplast RNA transcripts of vascular plants undergo C to U editing at approximately 30 sites, but there is no consensus sequence that identifies a C to be edited. Both sequences closely surrounding an edited C and unidentified site-specific trans-acting factors have been shown to be important for editing. The ability of an already edited transgenic sequence to bind and thus titrate a trans-acting editing factor was evaluated for two editing sites, ndhF and rpoB site 2. The U-containing rpoB transcripts did not affect editing of the endogenous rpoB transcripts, likely because the comparable C-containing transcripts containing 27 nucleotides surrounding the edited C were only 20% edited, indicating a low affinity of a trans-factor for this length of edited sequence. Surprisingly, U-containing ndhF transgene transcripts reduced endogenous ndhF transcript editing to the same degree as a C-containing transgene transcript. This indicates that the C target of editing is not a critical recognition feature for the site-specific trans-acting factor. (C) 2001 Elsevier Science B.V. All rights reserved.
