Effects of a Caenorhabditis elegans Dauer Pheromone Ascaroside on Physiology and Signal Transduction Pathways
Effects of a Caenorhabditis elegans Dauer Pheromone Ascaroside on Physiology and Signal Transduction Pathways
复制标题
DOI：
10.1007/s10886-009-9599-3
复制
发表时间：
2009-02-01
影响因子：
2.3
通讯作者：
Riddle, Donald L.
中科院分区：
文献类型：
--
作者：
Gallo, Marco;Riddle, Donald L.
Daumone 是秀丽隐杆线虫多尔信息素的三种纯化和人工合成成分之一。它影响已知区分 dauer 阶段发育停滞和成年生长的主要信号转导途径 [转化生长因子 β (TGF-β) 和 daf-2/IGF1R 途径]，就像天然信息素提取物一样。在前幼虫中，daf-7/TGF-β 的转录减少，而在暴露于合成道莫酮的胚胎和 L1 幼虫中，DAF-16/FOXO 转录因子的核定位增加。然而，daumone 不需要两栖神经元中的纤毛来产生这些作用，也不需要 G α 蛋白 GPA-3 来诱导 dauer 进入，尽管天然 dauer 信息素提取物诱导 dauer 需要 GPA-3。合成道莫酮具有天然信息素未观察到的生理作用。它在生物测定所需的浓度下是有毒的，并且对于角质层有缺陷的突变体是致命的。道尔酮和天然道尔信息素的分子和生理作用仅部分重叠。
Daumone is one of the three purified and artificially synthesized components of the Caenorhabditis elegans dauer pheromone. It affects the major signal transduction pathways known to discriminate between developmental arrest at the dauer stage and growth to the adult [the transforming growth factor beta (TGF-beta) and daf-2/IGF1R pathways], just as natural pheromone extracts do. Transcription of daf-7/TGF-beta is reduced in pre-dauer larvae, and nuclear localization of the DAF-16/FOXO transcription factor is increased in embryos and L1 larvae exposed to synthetic daumone. However, daumone does not require the cilia in the amphidial neurons to produce these effects nor does it require the G alpha protein GPA-3 to induce dauer entry, although GPA-3 is required for dauer induction by natural dauer pheromone extracts. Synthetic daumone has physiological effects that have not been observed with natural pheromone. It is toxic at the concentrations required for bioassay and is lethal to mutants with defective cuticles. The molecular and physiological effects of daumone and natural dauer pheromone are only partially overlapping.