Antibodies against heat shock protein 60 derived from Helicobacter pylori:: Diagnostic implications in cardiovascular disease
Antibodies against heat shock protein 60 derived from Helicobacter pylori:: Diagnostic implications in cardiovascular disease
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DOI：
10.1016/j.jaut.2007.05.004
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发表时间：
2007-09-01
影响因子：
12.8
通讯作者：
Oguma, Keiji
中科院分区：
文献类型：
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作者：
Okada, Tomoyuki;Ayada, Kiyoshi;Oguma, Keiji
针对病原体来源的热休克蛋白60（HSP 60）的免疫应答被认为是防御性事件，其由于分子模拟而误导至人类对应物。因此，动脉粥样硬化可以通过抗HSP 60抗体（Abs）进行血清学预测。在本研究中，我们分析了心血管疾病（CVD; n = 250）患者血清中抗幽门螺杆菌（Hp）衍生的HSP 60（Hp-HSP 60）或其肽段的IgG抗体的临床患病率，并与年龄和性别匹配的非CVD患者（n = 293）进行比较。抗Hp细胞裂解物抗体常出现在与CVD无关的Hp感染患者中。相反，针对特定氨基酸序列Hp-HSP 60（II 3）（Hp-HSP 60中的II 3区，Glu（141）-Leu（160））的Ab主要出现在CVD患者中，以及IgG抗人HSP 60（Hu-HSP 60（w））。抗Hp-HSP 60（113）和抗Hu-HSP 60（w）抗体滴度与hsCRP水平无相关性。提示Hp-HSP 60（Ⅱ 3）抗体与Hu-HSP 60（w）抗体交叉反应是心血管病的独立诊断标志物。此外，20个氨基酸残基（Glu（141）-Leu（160））可能是诱导抗Hu-HSP 60自身抗体的主要CVD相关表位，其位置在Hu-HSP 60的三级结构中被预测。(c)2007爱思唯尔有限公司版权所有。
Immune responses against heat shock protein 60 (HSP60) of pathogen-origin are thought to be defensive events which, due to molecular mimicry, misdirect to a human counterpart. Therefore, atherosclerosis may be serologically predicted by anti-HSP60 antibodies (Abs). In the present study, we analyzed the clinical prevalence of the serum IgG Abs against Helicobacter pylori (Hp)-derived HSP60 (Hp-HSP60) or its peptide fragments in patients with cardiovascular disease (CVD; n = 250), as compared to those in age- and gender-matched non-CVD patients (n = 293). Anti-Hp cell lysate Abs frequently appeared in Hp-infected patients who were not associated with CVD. In contrast, Abs against the particular amino acid sequence Hp-HSP60(II3) (II3 region, Glu(141)-Leu(160), in Hp-HSP60) predominantly appeared in CVD patients, as well as IgG anti-human HSP60 (Hu-HSP60(w)). Furthermore, neither titer of anti-Hp-HSP60(113) nor anti-Hu-HSP60(w) Abs was correlated with the levels of high sensitivity C-reactive protein (hsCRP). This data strongly suggested that IgG anti-Hp-HSP60(II3) Abs cross-reacted with Hu-HSP60(w) were independent diagnostic markers relevant to CVD. Further, the 20 amino acid residues (Glu(141)-Leu(160)) might be predominant CVD-associated epitopes that induce anti-Hu-HSP60 auto-Abs, whose location was predicted in the tertiary structure of Hu-HSP60. (c) 2007 Elsevier Ltd. All rights reserved.