A simple light-emitted diode-induced fluorescence detector using optical fibers and a charged coupled device for direct and indirect capillary electrophoresis methods
A simple light-emitted diode-induced fluorescence detector using optical fibers and a charged coupled device for direct and indirect capillary electrophoresis methods
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DOI：
10.1002/elps.200500701
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发表时间：
2006-05-01
期刊：
影响因子：
2.9
通讯作者：
Fernandez-Gutierrez, Alberto
中科院分区：
文献类型：
--
作者：
Arraez-Roman, David;Fernandez-Sanchez, Jorge F.;Fernandez-Gutierrez, Alberto
以蓝光二极管(470 Nm)为激发源，分叉光纤为光波导，CCD摄像机为检测器，构建了一种用于直接和间接毛细管电泳法的荧光检测器。所有部件的连接即使对于非专家来说也是相当容易的，与使用PMT的其他探测器相比，使用CCD摄像机提高了这种探测器的适用性，因为它允许记录二维电泳图或磷光测量。该检测器提供了一种紧凑、低成本和快速的系统，用于直接荧光检测的CE测定具有高量子产率的天然荧光化合物，荧光素的LOD为2.6×10(-6)M；FITC标记的1，6-二氨基己烷的荧光衍生化合物的CE直接荧光检测的LOD为1.6×10(-7)IM；和间接荧光检测的非荧光化合物的CE的LOD为2.7×10(-6)M。
We constructed a simple fluorescence detector for both direct and indirect CE methods using a blue light-emitted diode (470 nm) as excitation source, a bifurcated optical fiber as a waveguide, and a CCD camera as a detector. The connection of all the components is fairly easy even for nonexperts and the use of a CCD camera improves the applicability of this detector compared to the others using PMTs because it permits the recording of 2-D electropherograms or phosphorescence measurements. This detector provides a compact, low cost, and rapid system for the determination of native fluorescence compounds which have high quantum yields by CE with direct fluorescence detection, showing an LOD of 2.6 x 10(-6) M for fluorescein; the determination of fluorescence derivative compounds by CE with direct fluorescence detection, showing an LOD of 1.6 x 10(-7) IM for FITC-labeled 1,6-diaminohexane; and non-fluorescence compounds by CE with indirect fluorescence detection with an LOD of 2.7 x 10(-6) M for gallic acid.