FULL-TERM DEVELOPMENT OF MOUSE BLASTOMERE NUCLEI TRANSPLANTED INTO ENUCLEATED 2-CELL EMBRYOS
FULL-TERM DEVELOPMENT OF MOUSE BLASTOMERE NUCLEI TRANSPLANTED INTO ENUCLEATED 2-CELL EMBRYOS
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DOI：
10.1002/jez.1402420205
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发表时间：
1987-05-01
影响因子：
--
通讯作者：
SUGIE, T
中科院分区：
文献类型：
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作者：
TSUNODA, Y;YASUI, T;SUGIE, T
来自四细胞和八细胞小鼠胚胎的细胞核被移植到去核的两细胞胚胎中。研究发现，此类胚胎不仅在体外发育至囊胚阶段（72%和35%），而且在移植到受体小鼠体内后也发育至足月（22%和8%）。然而，没有观察到含有来自内细胞团的细胞核的胚胎的发育。由于含有高级细胞核的去核受精卵的发育受到限制（本研究；McGrath 和 Solter：Science，226：1317-1319，''84；Robl，Gilligan，Critser 和 First：Biol.Reprod.，34：733-739，''86），看来细胞质因子对于高级细胞的细胞核发育很重要。
The nuclei from four- and eight-cell mouse embryos were transplanted into enucleated two-cell embryos. It was found that such embryos not only developed to the blastocyst stage in vitro (72% and 35%), but also developed to full term (22% and 8%) after transfer to recipient mice. However, development of embryos which contained nuclei from the inner cell mass was not observed. Since the development of enucleated zygotes which contain advanced nuclei is limited (the present study; McGrath and Solter: Science, 226:1317-1319, ''84; Robl, Gilligan, Critser, and First: Biol. Reprod., 34:733-739, ''86), it appears that cytoplasmic factors are important for the development of nuclei from advanced cells.