Integration of single-cell trapping and impedance measurement utilizing microwell electrodes
Integration of single-cell trapping and impedance measurement utilizing microwell electrodes
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DOI：
10.1016/j.bios.2010.08.080
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发表时间：
2011-01-15
影响因子：
12.6
通讯作者：
Jang, Ling-Sheng
中科院分区：
文献类型：
--
作者：
Lan, Kung-Chieh;Jang, Ling-Sheng
研究单个细胞的能力在多种生物学研究中被认为很重要。在本研究中，细胞阻抗分析被集成到单细胞捕获结构中。为了实现精确定位，开发了一种细胞操纵和测量微芯片，它利用交流电热效应（ACET）和负介电电泳（nDEP）力来移动测量电极上的颗粒和细胞。 ACET 和 nDEP 可以轻松地与基于电场的后续分析结合起来。先前研究中提出的微孔被分成两部分，被视为测量电极。对原有结构进行改造，实现精确定位。进行数值模拟和分析来计算和分析结构参数的影响。模拟和分析的结果用于获得电池的最佳结构。微孔的捕获范围可以针对各种尺寸的细胞进行设计。为了证明定位的精度，对粒子进行了两次捕获、测量和释放。结果表明，颗粒的阻抗误差约为3%。最后，开发的结构被应用于捕获和测量 HeLa 细胞的阻抗。 (C) 2010 Elsevier B.V. 保留所有权利。
The ability to research individual cells has been seen as important in many kinds of biological studies. In the present study, cell impedance analysis is integrated into a single-cell trapping structure. For the purpose of precise positioning, a cell manipulation and measurement microchip, which uses an alternating current electrothermal effect (ACET) and a negative dielectrophoresis (nDEP) force to move a particle and cell on measurement electrodes, is developed. An ACET and an nDEP can be easily combined with subsequent analyses based on electric fields. A microwell presented in a previous study is separated into two parts, which are regarded as the measurement electrodes. The original structure is modified for precise positioning. Numerical simulations and analyses are conducted to compute and analyze the effects of the structural parameters. The results of simulations and analyses are used to obtain the optimum structure for the cell. The capture range of the microwell can be designed for cells of various sizes. In order to demonstrate the precision of the positioning, a particle is captured, measured, and released twice. The results show that the impedance error of the particle is about 3%. Finally, the developed structure is applied to trap and measure the impedance of a HeLa cell. (C) 2010 Elsevier B.V. All rights reserved.