Immunohistochemical study of renal fibropoiesis associated with dogs naturally and experimentally infected with two different strains of Leishmania (L.) infantum
Immunohistochemical study of renal fibropoiesis associated with dogs naturally and experimentally infected with two different strains of Leishmania (L.) infantum
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DOI：
10.1111/iep.12321
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发表时间：
2019-11-06
影响因子：
3
通讯作者：
Tafuri, Wagner L.
中科院分区：
文献类型：
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作者：
Alves, Adriano F.;Pereira, Ramon A.;Tafuri, Wagner L.
本工作的目的是研究自然感染婴儿利什曼原虫的狗和实验感染两种不同株L婴儿利什曼原虫的狗肾脏的某些病理变化，特别是纤维化过程。收集78例肾脏石蜡包埋标本如下：(A)CNI组，由62只成年犬肾脏标本组成，自然感染L幼犬；(B)BH401组，由5只实验感染L幼犬的成年比格犬肾脏标本组成；(C)BH400组，由11只实验感染L幼犬McAn/BR/2000/BH400的成年比格犬肾脏标本组成，按与前一组相同剂量、相同途径组成，命名为BH400组；对照组(CC组)，由4只成年比格犬肾脏标本组成。所有动物均表现出与不同类型的肾小球肾炎和慢性间质性肾炎相关的肾小球和间质纤维化。BH401组的纤维化明显更严重，其次是CNI组的动物。用免疫组织化学方法检测肌成纤维细胞间充质标志物α-肌动蛋白(α-SMA)、波形蛋白和细胞因子转化生长因子-β(TGF-β)的表达。BH401组上述指标的表达均高于其他组。细胞内无鞭毛体形式的利什曼原虫主要在BH401中发现。这些结果表明McAn/BR/2002/BH401株是研究肾脏LVC实验模型的良好选择。
The objectives of this work were to study some pathological aspects of kidneys obtained from dogs naturally infected with Leishmania infantum and from dogs experimentally infected with two different strains of L infantum with special emphasis on fibrotic process. Seventy eight specimens of paraffin-embedded kidney fragments were collected as follows: (a) CNI group composed by 62 kidney samples of adult mongrel dogs, naturally infected with L infantum; (b) BH401 group composed by five kidney samples of adult Beagles experimentally infected with L infantum strain MCAN BR/2002/BH401; (c) BH400 group composed by eleven kidney samples of adult Beagles experimentally infected with L infantum strain MCAN/BR/2000/BH400, at the same dose and same route of the previous group, denominated group BH400; Control group (CC) composed by four kidney samples of adult Beagles. All animals revealed glomerular and interstitial fibropoiesis associated with different types of glomerulonephritis and chronic interstitial nephritis. Fibrosis was markedly more intense in the BH401 group, followed by animals in the CNI group. Markers for myofibroblasts (mesenchymal markers) such as alpha-actin (alpha-SMA), vimentin and the cytokine transforming growth factor beta (TGF-beta) were done by immunohistochemistry. BH401 group showed higher expression of all these markers than others. Intracellular amastigotes forms of Leishmania was mainly found in BH401. These results could be indicating that the MCAN/BR/2002/BH401 strain is a good choice for the study of renal LVC experimental model.