Antiglomerular basement membrane antibody: antibody specificity in different forms of glomerulonephritis.
Antiglomerular basement membrane antibody: antibody specificity in different forms of glomerulonephritis.
复制标题
抗肾小球基底膜抗体：不同形式肾小球肾炎的抗体特异性。
作者：
J. Wieslander;P. Bygren;D. Heinegård
通过用胶原酶和胃蛋白酶消化或直接或在用酶消化之前用盐酸胍提取，从人肾小球基底膜溶解组分。不同的制剂被用作抗原，在酶联免疫吸附试验（ELISA）中的抗体滴度的患者与Goodpasture综合征和其他形式的肾小球肾炎，即系统性红斑狼疮，结节性动脉周围炎，IgA相关的肾病患者的血清中。Goodpasture综合征患者的IgG抗体滴度高，与胶原酶反应最强烈。盐酸胍提取仅部分溶解抗原，胃蛋白酶消化和还原可破坏抗原，胰蛋白酶消化可部分破坏抗原。抗原很可能是非胶原蛋白。来自其他形式肾炎患者的抗体主要针对盐酸胍提取物中的抗原，而抗原不被胶原酶消化溶解。胃蛋白酶消化破坏抗原。这些抗体属于不同的类别，即系统性红斑狼疮患者有IgG和伊加以及IgM抗体;结节性动脉周围炎患者有IgM或IgG和伊加抗体，而IgA相关肾炎患者的伊加滴度最高，但也有IgG和IgM抗体。没有一个患者有针对三螺旋胶原的抗体。因此，抗GBM抗体相关性肾炎的抗体应答在抗原和抗体类别方面是不同的，并且取决于潜在的疾病综合征。
Components were solubilized from human glomerular basement membrane by digestion with collagenase and pepsin or by extraction with guanidine-HCl either directly or after previous digestion with the enzyme. The diverse preparations were used as antigens in the enzyme-linked immunosorbent assay (ELISA) of antibody titers in sera from patients with Goodpasture syndrome and patients with other forms of glomerulonephritis, that is, systemic lupus erythematosus, periarteritis nodosa, and IgA-related nephropathy. Patients with Goodpasture syndrome had high titers of IgG antibodies reacting most strongly with collagenase digests. The antigen(s) was only partly solubilized by guanidine-HCl extraction, was destroyed by pepsin digestion as well as reduction, and partly destroyed by trypsin digestion. The antigen(s) is most likely noncollagenous protein. Antibodies from patients with other forms of nephritis were directed primarily against antigens in guanidine-HCl extracts, while the antigen(s) was not solubilized by collagenase digestion. Pepsin digestion destroyed the antigen(s). The antibodies were of a different class, that is, the patients with systemic lupus erythematosus had IgG and IgA as well as IgM antibodies; the patients with periarteritis nodosa had IgM or IgG and IgA antibodies, while the patients with IgA-related nephritis had the highest recorded titers of IgA but also had IgG as well as IgM antibodies. None of the patients had antibodies directed against triple helical collagen. The antibody response in anti-GBM antibody-related nephritis, then, is different both with respect to antigen and antibody class and depends on the underlying disease syndrome.