Production and Characterization of Monoclonal Strain‐Specific Antibodies against Prototype Strains of Rickettsia tsutsugamushi
Production and Characterization of Monoclonal Strain‐Specific Antibodies against Prototype Strains of Rickettsia tsutsugamushi
复制标题
针对恙虫病立克次体原型菌株的单克隆菌株特异性抗体的制备和表征
DOI：
10.1111/j.1348-0421.1986.tb02987.x
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发表时间：
1986
影响因子：
2.6
通讯作者：
A. Kawamura
中科院分区：
文献类型：
--
作者：
M. Murata;Y. Yoshida;M. Osono;N. Ohashi;M. Oyanagi;H. Urakami;A. Tamura;S. Nogami;Hiroshi Tanaka;A. Kawamura
我们开发了18种杂杂瘤细胞系，这些细胞系可分泌针对恙虫病立克次体原型株的小鼠单克隆株特异性抗体：9种抗Gilliam抗体，4种抗Karp抗体和5种抗Kato抗体。所有单克隆抗体仅与同源株直接、间接免疫荧光（IF）或间接免疫过氧化物酶（IP）反应。用单克隆抗体进行IF和IP检测，从日本螨类、田鼠和恙虫病（恙虫病）患者中分离到的22株恙虫体均明确为Gilliam型或Karp型。聚丙烯酰胺凝胶电泳和免疫印迹技术分析表明，单克隆抗体主要识别同源立克次体表面表观分子量为54 ~ 56千道尔顿的多肽。本研究中产生的单克隆抗体可以识别菌株或类型特异性多肽，并且在菌株之间没有交叉反应，因此可以加强立克次体抗原的血清分型和进一步的分析研究。
We have developed 18 hybridoma cell lines which secrete murine monoclonal strain‐specific antibodies to prototype strains of Rickettsia tsutsugamushi: nine anti‐Gilliam, four anti‐Karp and five anti‐Kato antibodies. All the monoclonal antibodies reacted only with their homologous strains in direct and indirect immunofluorescence (IF), or indirect immunoperoxidase (IP) test. By IF and IP tests with the monoclonal antibodies, 22 strains of R. tsutsugamushi, which were newly isolated from mites, field rodents and patients with Tsutsugamushi disease (scrub typhus) in Japan, were all clearly identified as either Gilliam or Karp type. Analysis by polyacrylamide gel electrophoresis and immunoblotting techniques revealed that the monoclonal antibodies recognized primarily the polypeptides of an apparent molecular weight of 54 to 56 kilodaltons of the homologous rickettsial surface. The monoclonal antibodies produced in the present study should enhance the serotyping and further analytical investigation of the rickettsial antigens since they recognize the strain‐ or type‐specific polypeptides and do not show any cross‐reaction among strains.