Hidden totipotency in naïve human pluripotent stem cell cultures.
Hidden totipotency in naïve human pluripotent stem cell cultures.
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DOI：
10.1093/lifemedi/lnac024
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发表时间：
2022-12
期刊：
影响因子：
--
通讯作者：
Wu, Jun
中科院分区：
文献类型：
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作者：
Schmitz, Daniel A;Wu, Jun
对人类早期胚胎发生的了解是开发人类发育疾病和不孕不育治疗方法的关键，但它受到伦理、法律和技术限制的阻碍，这些限制涉及使用实际人类胚胎进行研究。正因为如此，研究人员开发了各种人类早期胚胎发生的体外模型，作为人类胚胎的可获得性替代品。早期胚胎建模领域的主力一直是多能干细胞(PSC)。这些细胞具有非凡的能力，可以在成人体内产生任何细胞，并可以在培养中无限自我更新。在胚胎发生的背景下，PSCs作为多能上胚层的体外表达，产生胚胎本身，而不是胚外组织。因此，一般而言，PSCs缺乏形成胚外组织的发育能力。由于这一局限性，近年来人们做出了很大努力来培养出比PSCs具有更大发育能力的体外细胞系：全能干细胞。
The understanding of human early embryogenesis is key to developing treatments for human developmental disease and infertility, but it has been hampered by ethical, legal, and technical limitations involving research using actual human embryos. Because of this, researchers have developed various in vitro models of early human embryogenesis to act as accessible proxies for human embryos.The workhorse of the early embryo modeling field has been the pluripotent stem cell (PSC). These cells have the remarkable ability to give rise to any cell in the adult body and can self-renew indefinitely in culture. In the context of embryogenesis, PSCs act as the in vitro representation of the pluripotent epiblast which gives rise to the embryo proper but not extraembryonic tissues. Thus, in general, PSCs lack the developmental capacity to give rise to extraembryonic tissues. Because of this limitation, a great effort has been made in recent years to generate in vitro cell lines that have an even greater developmental capacity than that of PSCs: totipotent stem cells.