Enhanced Function of Chondrocytes in a Chitosan-based Hydrogel to Regenerate Cartilage Tissues by Accelerating Degradability of the Hydrogel via a Hydrolysable Cross-Linker
Enhanced Function of Chondrocytes in a Chitosan-based Hydrogel to Regenerate Cartilage Tissues by Accelerating Degradability of the Hydrogel via a Hydrolysable Cross-Linker
复制标题
通过可水解交联剂加速水凝胶的降解性，增强基于壳聚糖的水凝胶中软骨细胞再生软骨组织的功能
DOI：
10.1002/app.48893
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
and Hidenori Otsuka
中科院分区：
文献类型：
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作者：
Shohei Ishikawa;Kazutoshi Iijima;Daisuke Matsukuma;Michihiro Iijima;Shigehito Osawa;and Hidenori Otsuka
使用具有和不具有可水解聚（dl-丙交酯）（PLA）片段的连接体制备作为用于培养软骨细胞的支架的壳聚糖基水凝胶。对其中培养的软骨细胞的评价表明，通过PLA水解的水凝胶加速降解略微促进了硫酸化糖胺聚糖的产生，并显著改善了来自包封的软骨细胞的胶原蛋白的产生，所述软骨细胞是软骨特异性细胞外基质组分。此外，加速的降解性显著上调用于II型胶原蛋白产生的基因表达，并下调用于包封的软骨细胞的I型胶原蛋白产生的基因表达。由于关节软骨组织的主要成分是富含II型胶原的透明软骨，因此这些结果表明支架的降解是软骨组织再生的重要参数，加速降解可能有利于促进软骨组织再生，特别是从透明软骨样胶原产生的角度来看。© 2019 Wiley Periodicals，Inc. J.应用聚合物Sci.2019，137，48893.
Chitosan‐based hydrogels as scaffolds for culturing chondrocytes were prepared using linkers with and without hydrolysable poly(dl‐lactide) (PLA) segments. The evaluation of the cultured chondrocytes in them indicated that the accelerated degradation of the hydrogel via hydrolysis of the PLA slightly promoted production of the sulfated glycosaminoglycan and drastically improved that of collagen from the encapsulated chondrocytes, which are the chondrospecific extracellular matrix components. Furthermore, the accelerated degradability significantly upregulated the gene expression for Collagen II production and downregulated that for Collagen I production of the encapsulated chondrocytes. Because major component of the articular cartilage tissue is Collagen II‐rich hyaline cartilage, these results suggest the degradation of the scaffolds is an important parameter in cartilage tissue regeneration and the accelerated degradability may have benefits on promotion of cartilage tissue regeneration especially from the viewpoint of hyaline cartilage‐like collagen production. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci.2019,137, 48893.