Skeletal muscle capillary density and microvascular function are compromised with aging and type 2 diabetes
Skeletal muscle capillary density and microvascular function are compromised with aging and type 2 diabetes
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DOI：
10.1152/japplphysiol.00919.2013
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发表时间：
2014-04-01
影响因子：
3.3
通讯作者：
van Loon, Luc J. C.
中科院分区：
文献类型：
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作者：
Groen, Bart B. L.;Hamer, Henrike M.;van Loon, Luc J. C.
维持骨骼肌质量需要足够的肌肉灌注。微血管结构和/或功能与衰老和2型糖尿病的损害与骨骼肌质量的进行性损失有关。我们的目的是比较健康年轻男性、健康老年男性和年龄匹配的2型糖尿病患者之间的肌纤维类型特异性毛细血管密度和内皮功能。选择15名健康年轻男性（24 +/- 1岁）、15名健康老年男性（70 +/- 2岁）和15名年龄匹配的2型糖尿病患者（70 +/- 1岁）参与本研究。评估全身胰岛素敏感性、肌纤维类型特异性毛细血管密度、舌下微血管密度和红细胞灌注边界区域的尺寸，以评价衰老和/或2型糖尿病对微血管结构和功能的影响。全身胰岛素敏感性在更高的年龄显著降低，2型糖尿病患者报告的值最低。与年轻人相比，老年人和老年2型糖尿病患者的骨骼肌毛细血管接触要低得多。侧流暗视野成像显示2型糖尿病患者红细胞灌注边界区的厚度明显大于年轻人。骨骼肌毛细血管密度随着年龄增长和2型糖尿病而降低，并伴有内皮糖萼功能受损，这表明血管功能受损。
Adequate muscle perfusion is required for the maintenance of skeletal muscle mass. Impairments in microvascular structure and/or function with aging and type 2 diabetes have been associated with the progressive loss of skeletal muscle mass. Our objective was to compare muscle fiber type specific capillary density and endothelial function between healthy young men, healthy older men, and age-matched type 2 diabetes patients. Fifteen healthy young men (24 +/- 1 yr), 15 healthy older men (70 +/- 2 yr), and 15 age-matched type 2 diabetes patients (70 +/- 1 yr) were selected to participate in the present study. Whole body insulin sensitivity, muscle fiber type specific capillary density, sublingual microvascular density, and dimension of the erythrocyte-perfused boundary region were assessed to evaluate the impact of aging and/or type 2 diabetes on microvascular structure and function. Whole body insulin sensitivity was significantly lower at a more advanced age, with lowest values reported in the type 2 diabetic patients. In line, skeletal muscle capillary contacts were much lower in the older and older type 2 diabetic patients when compared with the young. Sidestream darkfield imaging showed a significantly greater thickness of the erythrocyte perfused boundary region in the type 2 diabetic patients compared with the young. Skeletal muscle capillary density is reduced with aging and type 2 diabetes and accompanied by impairments in endothelial glycocalyx function, which is indicative of compromised vascular function.