A XANTHOMONAS-CAMPESTRIS PV CAMPESTRIS PROTEIN SIMILAR TO CATABOLITE ACTIVATION FACTOR IS INVOLVED IN REGULATION OF PHYTOPATHOGENICITY
A XANTHOMONAS-CAMPESTRIS PV CAMPESTRIS PROTEIN SIMILAR TO CATABOLITE ACTIVATION FACTOR IS INVOLVED IN REGULATION OF PHYTOPATHOGENICITY
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DOI：
10.1128/jb.172.10.5877-5883.1990
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发表时间：
1990-10-01
影响因子：
3.2
通讯作者：
DANCHIN, A
中科院分区：
文献类型：
--
作者：
DECRECYLAGARD, V;GLASER, P;DANCHIN, A
野油菜黄单胞菌致病变种（Xanthomonas campestris pv.）campestris部分恢复了cya crp大肠杆菌菌株的碳水化合物发酵模式，并在E.杆菌该片段的核苷酸序列显示存在一个700个碱基对的开放阅读框架，其编码的蛋白与E.大肠杆菌（相应地命名为CLP，表示CAP样蛋白）。X野油菜致病变种通过反向遗传学方法构建了白菜型clp突变体。该菌株对各种碳源的利用不受影响，但其致病性明显降低。黄原胶，色素和胞外酶的生产增加或减少，表明CLP起着调节植物病原性的作用。
A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein highly similar to the catabolite activation factor (CAP) of E. coli (accordingly named CLP for CAP-like protein). An X. campestris pv. campestris clp mutant was constructed by reverse genetics. This strain was not affected in the utilization of various carbon sources but had strongly reduced pathogenicity. Production of xanthan gum, pigment, and extracellular enzymes was either increased or decreased, suggesting that CLP plays a role in the regulation of phytopathogenicity.