Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry
Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry
复制标题
使用质谱法对不同乳腺癌亚型中甘露糖受体的完整糖肽进行比较分析。
DOI：
10.1016/j.talanta.2020.121676
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发表时间：
2021-02-01
期刊：
影响因子：
6.1
通讯作者：
Lu, Haojie
中科院分区：
文献类型：
--
作者：
Fang, Jing;Sheng, Xiangying;Lu, Haojie
乳腺癌是一种高度异质性的疾病，包括许多具有特定病理特征和生物学行为的不同生物学实体。在初步实验中，我们确定了不同乳腺癌亚型中甘露糖受体的几个糖基化位点，并表明甘露糖受体可能是乳腺癌的潜在标记物。然而，由于PNGase F在以前的工作中移除了每个位置的多糖，因此每个位置上的多糖组成仍然未知。对完整糖肽的分析可以提供糖链组成和糖基化位点的信息，从而有助于揭示乳腺癌四种亚型的糖基化差异。本工作利用相对和绝对定量等压标签(ITRAQ)和LC-MS/MS分析了乳腺癌患者血清中甘露糖受体的完整糖肽。从四种乳腺癌亚型中共鉴定出26个完整糖肽对应的7个糖基化位点和12种糖类。其中11个糖肽可用于乳腺癌亚型的鉴别，进一步支持了甘露糖受体可作为乳腺癌亚型鉴别的潜在标记物的结论。
Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors. In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer. However, the glycan composition on each site is still unknown because the glycan was removed by PNGase F in previous work. Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer. In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS. In total, 7 glycosylation sites and 12 glycan types corresponding to 26 intact glycopeptides were characterized from the four subtypes of breast cancer. Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.