EVIDENCE FOR TEKTINS IN CENTRIOLES AND AXONEMAL MICROTUBULES
EVIDENCE FOR TEKTINS IN CENTRIOLES AND AXONEMAL MICROTUBULES
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DOI：
10.1073/pnas.85.8.2643
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发表时间：
1988-04-01
影响因子：
11.1
通讯作者：
LINCK, RW
中科院分区：
文献类型：
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作者：
STEFFEN, W;LINCK, RW
从海胆精子轴突微管中分离得到三种tektin (tektin， A， B， C)，制备了亲和纯化的多克隆抗体。这些抗体（抗tektin）用于定位轴突体、基底体和中心粒中的tektin。免疫荧光显微镜显示，在Lytechinus pictus精子尾部，这三种抗tektin在9个轴突双微管和沿其全长延伸的a小管中都有染色。除了染色双重微管外，抗tektin C还弱标记了小红花精尾的中央对微管。抗tektin染色还显示与海胆精子基底体和仓鼠、人类和猪细胞的中心粒有明显的交叉反应。这些数据提供了在基底体和中心粒中存在tektin或tektin样蛋白的证据，并表明中心粒微管的构造原理与鞭毛微管相同。
Affinity purified, polyclonal antibodies were prepared against three tektins (tektins, A, B, and C) isolated from sea urchin sperm axonemal microtubules. These antibodies (anti-tektins) were used to localize tektins in axonemes, basal bodies, and centrioles. By immunofluorescence microscopy it could be demonstrated that in sperm tails from Lytechinus pictus all three anti-tektins stain in nine axonemal doublet microtubles and A-tubule extensions along their entire length. In addition to staining doublet microtubules, anti-tektin C weakly labeled the central-pair microtubules in sperm tails from Patiria miniata. The anti-tektin staining revealed also a clear cross-reaction with basal bodies of sea urchin sperm and with centrioles of cells from hamsters, humans, and pigs. These data provide evidence of tektin or tektin-like proteins in basal bodies and centrioles and suggest that centriole microtubules are constructed according to the same principles as flagellar microtubules.