RNF17, a component of the mammalian germ cell nuage, is essential for spermiogenesis
RNF17, a component of the mammalian germ cell nuage, is essential for spermiogenesis
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DOI：
10.1242/dev.02003
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发表时间：
2005-09-01
期刊：
影响因子：
4.6
通讯作者：
Wang, PJ
中科院分区：
文献类型：
--
作者：
Pan, JY;Goodheart, M;Wang, PJ
在各种生物体的生殖细胞中发现了营养素。然而，对小鼠出生后雄性生殖细胞中的nuages的研究却很少。在此之前，我们克隆了一个生殖细胞特异性基因Rnf 17，它编码一种含有RING指和Tudor结构域的蛋白质。在这里，我们报告说，RNF 17是一个新的nuage在男性生殖细胞的组成部分-RNF 17颗粒，这是一个电子致密的非膜结合的球形细胞器，直径为0.5 μ m。RNF 17颗粒在晚粗线期和双线期精母细胞以及伸长的精子细胞中突出。RNF 17颗粒可与其他已知的微粒子如拟染色体体区分开。RNF 17能够在体外和体内形成二聚体或聚合物，表明它可能在RNF 17颗粒的组装中起作用。Rnf 17缺陷的雄性小鼠不育，并表现出完全逮捕圆形精子细胞，表明Rnf 17编码一种新的关键调节精子发生。rnf 17-null圆形精子细胞进入第4步，但不能产生精子。这些结果表明，RNF 17是一种新的生殖细胞nuage的组分，是雄性生殖细胞分化所必需的。
Nuages are found in the germ cells of diverse organisms. However, nuages in postnatal male germ cells of mice are poorly studied. Previously, we cloned a germ cell-specific gene named Rnf17, which encodes a protein containing both a RING finger and tudor domains. Here, we report that RNF17 is a component of a novel nuage in male germ cells - the RNF17 granule, which is an electron-dense non-membrane bound spherical organelle with a diameter of 0.5 mu m. RNF17 granules are prominent in late pachytene and diplotene spermatocytes, and in elongating spermatids. RNF17 granules are distinguishable from other known nuages, such as chromatoid bodies. RNF17 is able to form dimers or polymers both in vitro and in vivo, indicating that it may play a role in the assembly of RNF17 granules. Rnf17-deficient male mice were sterile and exhibited a complete arrest in round spermatids, demonstrating that Rnf17 encodes a novel key regulator of spermiogenesis. Rnf17-null round spermatids advanced to step 4 but failed to produce sperm. These results have shown that RNF17 is a component of a novel germ cell nuage and is required for differentiation of male germ cells.