Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR
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DOI：
10.1128/jb.179.17.5636-5638.1997
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发表时间：
1997-09-01
影响因子：
3.2
通讯作者：
Errington, J
中科院分区：
文献类型：
--
作者：
Daniel, RA;Haiech, J;Errington, J
我们已经分离出编码枯草芽孢杆菌内源性β-半乳糖苷酶的lacA基因中的转座子插入。在含有LacA的可能操纵子的上游是一个负调控因子LACR，它编码一种与一系列调节因子相关的产物，其中包括大肠杆菌的乳糖抑制因子lac1。插入了lacA基因的新菌株应该会在枯草杆菌中使用lacZ融合的研究中使用。
We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lac1, of Escherichia coli. New strains with insertions in the lacA gene should be of use in studies using lacZ fusions in B. subtilis.